Abstract |
Hundreds of genes can be regulated by hypoxia-inducible factor 1 (HIF1) under hypoxia. Here we demonstrated a HIF1-mediated induction of protein phosphatase 1, regulatory subunit 3C gene (PPP1R3C) in human MCF7 cells under hypoxia. By mutation analysis we confirmed the presence of a functional hypoxia response element that is located 229bp upstream from the PPP1R3C gene. PPP1R3C induction correlates with a significant glycogen accumulation in MCF7 cells under hypoxia. Knockdown of either HIF1α or PPP1R3C attenuated hypoxia-induced glycogen accumulation significantly. Knockdown of HIF2α reduced hypoxia-induced glycogen accumulation slightly (but not significantly). Our results demonstrated that HIF1 promotes glycogen accumulation through regulating PPP1R3C expression under hypoxia, which revealed a novel metabolic adaptation of cells to hypoxia.
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Authors | Guo-Min Shen, Feng-Lin Zhang, Xiao-Ling Liu, Jun-Wu Zhang |
Journal | FEBS letters
(FEBS Lett)
Vol. 584
Issue 20
Pg. 4366-72
(Oct 22 2010)
ISSN: 1873-3468 [Electronic] England |
PMID | 20888814
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Carrier Proteins
- Hypoxia-Inducible Factor 1, alpha Subunit
- Intracellular Signaling Peptides and Proteins
- PPP1R3C protein, human
- Glycogen
- Phosphoprotein Phosphatases
- Oxygen
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Topics |
- Base Sequence
- Blotting, Western
- Carrier Proteins
(genetics, metabolism)
- Cell Hypoxia
- Cell Line, Tumor
- Gene Expression Regulation, Neoplastic
(drug effects)
- Glycogen
(metabolism)
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
(genetics, metabolism)
- Intracellular Signaling Peptides and Proteins
- Oxygen
(pharmacology)
- Phosphoprotein Phosphatases
(genetics, metabolism)
- Promoter Regions, Genetic
(genetics)
- RNA Interference
- Response Elements
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
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