Sairei-to ameliorates rat peritoneal fibrosis partly through suppression of oxidative stress.

Abstract

BACKGROUND:

Sairei-to is a herbal prescription originating from traditional Chinese medicine. We conducted an experimental study on rat peritoneal fibrosis to clarify the suppressive mechanisms of sairei-to.

METHODS:

Wistar rats were intraperitoneally injected with chlorhexidine gluconate (CG) every day. Peritoneal specimens were collected after 28 days of CG injection and oral administration of sairei-to. Macrophage infiltration, extracellular matrix accumulation, and angiogenesis were evaluated by immunostaining for ED-1, fibronectin, and CD-31, respectively. To observe oxidative stress in the tissue, 4-hydroxy-2-noneal (HNE) accumulation and plasma levels of superoxide dismutase (SOD) activity were detected. As a candidate of antioxidative components in sairei-to, plasma levels of baicalin were determined by high-performance liquid chromatography.

RESULTS:

Compared with the disease control group, serum total protein levels were significantly recovered in the sairei-to treatment group. Thickness of the submesothelial compact zone, trichrome-stained area, ED-1-positive cells, fibronectin-staining area, and HNE accumulation were suppressed in the treatment group. Concurrently, decreased plasma levels of SOD activity were recovered by sairei-to treatment. Increased CD-31-positive vessel number and area were also suppressed in the sairei-to group. Baicalin was detected in the plasma samples of the sairei-to group at 0.29 ± 0.11 μg/ml (mean±SEM).

CONCLUSION:

These results suggest that sairei-to ameliorates peritoneal fibrosis, partly through suppressing oxidative stress and macrophage infiltration.

• Authors: Masayuki Kitamoto, Kozue Kato, Akihisa Sugimoto, Hisayo Kitamura, Kazuhide Uemura, Toshiya Takeda, Chunmo Wu, Fumiaki Nogaki, Tatsuya Morimoto, Takahiko Ono
• Journal: Nephron. Experimental nephrology (Nephron Exp Nephrol) Vol. 117 Issue 3 Pg. e71-81 ( 2011) ISSN: 1660-2129 [Electronic] Switzerland
• PMID: 20881432 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 S. Karger AG, Basel.

Chemical References

• Drugs, Chinese Herbal
• Flavonoids
• Platelet Endothelial Cell Adhesion Molecule-1
• sairei-to
• baicalin
• Superoxide Dismutase

Topics

• Animals
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (therapeutic use)
• Flavonoids (blood)
• Male
• Neovascularization, Pathologic (pathology)
• Oxidative Stress (drug effects)
• Peritoneal Fibrosis (drug therapy, pathology)
• Platelet Endothelial Cell Adhesion Molecule-1 (analysis)
• Rats
• Rats, Wistar
• Superoxide Dismutase (blood)