Abstract | PURPOSE: The aim of this study was to investigate the protective effects of the flavonoid fraction of Moringa oleifera leaves (FMO) on selenite cataract in vivo. METHODS: RESULTS: The total phenolic content of FMO was 4.4 mg of catechin equivalent/g dried plant material. The extract showed remarkable activity on 2,2-diphenyl-picrylhydrazyl (IC50 36 µg/mL) and in superoxide radical (IC50 33.81µg/mL) scavenging assays. FMO effectively prevented the morphological changes and oxidative damage in lens. FMO maintained the activities of antioxidant enzymes and sulfhydryl content and prevented reactive oxygen species generation and lipid peroxidation. CONCLUSIONS: FMO was effective in preventing cataractogenesis in selenite model by enhancing the activities of antioxidant enzyme, reducing the intensity of lipid peroxidation, and inhibiting free radical generation.
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Authors | V Sasikala, B N Rooban, S G Siva Priya, V Sahasranamam, Annie Abraham |
Journal | Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
(J Ocul Pharmacol Ther)
Vol. 26
Issue 5
Pg. 441-7
(Oct 2010)
ISSN: 1557-7732 [Electronic] United States |
PMID | 20879807
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Flavonoids
- Free Radical Scavengers
- Phenols
- Plant Preparations
- Polyphenols
- Reactive Oxygen Species
- Thiobarbituric Acid Reactive Substances
- Catalase
- Glutathione Peroxidase
- Superoxide Dismutase
- Glutathione
- Sodium Selenite
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Topics |
- Animals
- Antioxidants
(administration & dosage, metabolism, pharmacology)
- Catalase
(analysis, metabolism, pharmacology)
- Cataract
(chemically induced, metabolism, prevention & control)
- Flavonoids
(analysis)
- Free Radical Scavengers
(analysis)
- Glutathione
(metabolism, pharmacology)
- Glutathione Peroxidase
(analysis, metabolism, pharmacology)
- Lipid Peroxidation
(drug effects)
- Moringa oleifera
- Oxidative Stress
(drug effects)
- Phenols
(analysis)
- Phytotherapy
- Plant Leaves
- Plant Preparations
(adverse effects, pharmacology)
- Polyphenols
- Rats
- Rats, Sprague-Dawley
- Reactive Oxygen Species
(analysis)
- Sodium Selenite
(metabolism, pharmacology)
- Superoxide Dismutase
(analysis, metabolism, pharmacology)
- Thiobarbituric Acid Reactive Substances
(analysis)
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