Ethyl pyruvate has anti-inflammatory and delayed myocardial protective effects after regional ischemia/reperfusion injury.

Abstract

PURPOSE:

Ethyl pyruvate has anti-inflammatory properties and protects organs from ischemia/reperfusion (I/R)-induced tissue injury. The aim of this study was to determine whether ethyl pyruvate decreases the inflammatory response after regional I/R injury and whether ethyl pyruvate protects against delayed regional I/R injury in an in vivo rat heart model after a 24 hours reperfusion.

MATERIALS AND METHODS:

Rats were randomized to receive lactated Ringer's solution or ethyl pyruvate dissolved in Ringer's solution, which was given by intraperitoneal injection 1 hour prior to ischemia. Rats were subjected to 30 min of ischemia followed by reperfusion of the left coronary artery territory. After a 2 hours reperfusion, nuclear factor κB, myocardial myeloperoxidase activity, and inflammatory cytokine levels were determined. After the 24 hours reperfusion, the hemodynamic function and myocardial infarct size were evaluated.

RESULTS:

At 2 hours after I/R injury, ethyl pyruvate attenuated I/R-induced nuclear factor κB translocation and reduced myeloperoxidase activity in myocardium. The plasma circulating levels of inflammatory cytokines decreased significantly in the ethyl pyruvate-treated group. At 24 hours after I/R injury, ethyl pyruvate significantly improved cardiac function and reduced infarct size after regional I/R injury.

CONCLUSION:

Ethyl pyruvate has the ability to inhibit neutrophil activation, inflammatory cytokine release, and nuclear factor κB translocation. Ethyl pyruvate is associated with a delayed myocardial protective effect after regional I/R injury in an in vivo rat heart model.

• Authors: In-Seok Jang, Mi-Young Park, Il-Woo Shin, Ju-Tae Sohn, Heon-Keun Lee, Young-Kyun Chung
• Journal: Yonsei medical journal (Yonsei Med J) Vol. 51 Issue 6 Pg. 838-44 (Nov 2010) ISSN: 1976-2437 [Electronic] Korea (South)
• PMID: 20879048 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• NF-kappa B
• Pyruvates
• ethyl pyruvate
• Peroxidase

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cell Nucleus (metabolism)
• Cytoplasm (metabolism)
• Heart (physiopathology)
• Inflammation
• Male
• Myocardial Infarction (prevention & control)
• Myocardium (metabolism)
• NF-kappa B (metabolism)
• Peroxidase (metabolism)
• Pyruvates (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy, metabolism)