Abstract |
CEP-18770, [(1R)-1-{[(2S,3R)-3-hydroxy-2-{[(6-phenyl-2-pyridinyl)carbonyl]amino}butanoyl]amino}-3-methylbutyl] boronic acid, is a novel proteasome inhibitor, now under early clinical evaluation as an anticancer agent. To investigate its clinical pharmacokinetics, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated to measure the drug in human plasma, based on simple protein precipitation with acetonitrile after the addition of irbesartan as internal standard. The method requires a small volume of sample (100 µl) and is rapid and selective, allowing good resolution of peaks in 5 min. It is sensitive, precise and accurate, with overall precision, expressed as coefficient of variation (CV%), always < 10.0%, accuracy in the range 93.8-107.7% and high recovery, close to 100%. The limit of detection is 0.01 ng/ml and the lower limit of quantitation (LLOQ) is 0.20 ng/ml. The assay was validated in the range from the LLOQ up to 50.00 ng/ml. This is the first method developed and validated for analyzing a proteasome inhibitor with a boronic-acid-based structure in human plasma. The method was successfully applied to study the pharmacokinetics of CEP-18770 in cancer patients with solid tumors or multiple myeloma who had received the drug as a short intravenous bolus during the initial Phase I trial.
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Authors | Federica Sala, Elena Marangon, Renzo Bagnati, Valeria Livi, Roberta Cereda, Maurizio D'Incalci, Massimo Zucchetti |
Journal | Journal of mass spectrometry : JMS
(J Mass Spectrom)
Vol. 45
Issue 11
Pg. 1299-305
(Nov 2010)
ISSN: 1096-9888 [Electronic] England |
PMID | 20872901
(Publication Type: Journal Article)
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Copyright | Copyright © 2010 John Wiley & Sons, Ltd. |
Chemical References |
- Acetonitriles
- Biphenyl Compounds
- Boronic Acids
- Proteasome Inhibitors
- Tetrazoles
- Threonine
- delanzomib
- Irbesartan
- acetonitrile
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Topics |
- Acetonitriles
(chemistry)
- Biphenyl Compounds
(analysis, chemistry)
- Boronic Acids
(blood, chemistry, pharmacokinetics)
- Chromatography, High Pressure Liquid
(methods)
- Clinical Trials, Phase I as Topic
- Drug Stability
- Humans
- Irbesartan
- Multiple Myeloma
- Neoplasms
- Proteasome Inhibitors
- Reproducibility of Results
- Sensitivity and Specificity
- Tandem Mass Spectrometry
(methods)
- Tetrazoles
(analysis, chemistry)
- Threonine
(analogs & derivatives, blood, chemistry, pharmacokinetics)
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