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Rapid resolution liquid chromatography (RRLC) analysis and studies on the stability of Shuang-Huang-Lian preparations.

Abstract
Shuang-Huang-Lian (SHL) is a traditional Chinese formula which comprises of three medicinal herbs: Flos Lonicerae, Radix Scutellariae and Fructus Forsythiae, and is commonly used to treat acute upper respiratory tract infection, acute bronchitis and light pneumonia. A simple, reliable and reproducible rapid resolution liquid chromatography (RRLC) method was developed for the quality control of SHL preparations, which baseline separates the major bioactive compounds within 6min. The method uses a C18-HST column (2.5μm, 100mm×3.0mm) kept at 40°C. The mobile phases consist of 0.1% phosphoric acid aqueous solution and acetonitrile. Flow rate is 1.0ml/min and UV detection is performed at 327nm from 0 to 4min and 229nm from 4 to 7min. This method was further validated according to the ICH guidelines. Eight batches of commercial SHL preparations obtained from different pharmaceutical manufacturers as well as individual herbs were examined and their chromatographic profiles were compared. The stability test revealed that chlorogenic acid is stable only at acidic pH, and hence it is necessary to further evaluate and optimize the preparatory procedures and storage conditions for commercial SHL preparations.
AuthorsYuan-Chun Ma, Xiao-Qiang Wang, Feifei Hou, Jie Ma, Mai Luo, Alice Chen, Peter Jin, Shane Lu, Iris Xu
JournalJournal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 54 Issue 2 Pg. 265-72 (Jan 25 2011) ISSN: 1873-264X [Electronic] England
PMID20855176 (Publication Type: Comparative Study, Journal Article)
CopyrightCopyright © 2010 Elsevier B.V. All rights reserved.
Chemical References
  • Drugs, Chinese Herbal
  • Chlorogenic Acid
Topics
  • Calibration
  • Chemistry, Pharmaceutical (standards)
  • Chlorogenic Acid (analysis)
  • Chromatography, Liquid (methods)
  • Drug Stability
  • Drugs, Chinese Herbal
  • Forsythia (chemistry)
  • Guidelines as Topic (standards)
  • Humans
  • Hydrogen-Ion Concentration
  • Limit of Detection
  • Lonicera (chemistry)
  • Medicine, Chinese Traditional
  • Molecular Structure
  • Plants, Medicinal (chemistry)
  • Quality Control
  • Reference Standards
  • Reproducibility of Results
  • Scutellaria baicalensis (chemistry)
  • Sensitivity and Specificity
  • Time Factors

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