Abstract | AIM: METHODS: Rats were divided into four groups: UUO with vehicle or tranilast and sham operation with vehicle or tranilast. Tranilast (400 mg/kg/day) was administrated to rats for 7 and 14 days after UUO. RESULTS:
Fibrosis and tubular injuries were attenuated in UUO kidneys with tranilast (Tr-UUO kidneys) compared with UUO kidneys with vehicle (V-UUO kidneys). Decreased E-cadherin and increased vimentin expression in the tubular epithelium and Snail expression in V-UUO kidneys were also attenuated in Tr-UUO kidneys in which heparan sulfate proteoglycan in the tubular basement membrane was preserved and matrix metalloproteinase-2 expression was attenuated. Increased TGF-β1 and phospho-Smad2 expression and increased numbers of myofibroblasts and macrophages in V-UUO kidneys were attenuated by tranilast. Decreased VE-cadherin expression and cytoplasmic swelling of the endothelium of peritubular capillaries that occurred in V-UUO kidneys was prevented by tranilast. CONCLUSIONS:
Tranilast modulates fibrogenesis by reducing EMT, preventing disintegration of the tubular basement membrane, and reducing peritubular capillary injury in UUO kidneys.
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Authors | Tomoki Kaneyama, Satoshi Kobayashi, Daiju Aoyagi, Takashi Ehara |
Journal | Pathology
(Pathology)
Vol. 42
Issue 6
Pg. 564-73
( 2010)
ISSN: 1465-3931 [Electronic] England |
PMID | 20854076
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- ortho-Aminobenzoates
- tranilast
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(pharmacology)
- Blotting, Western
- Capillaries
(injuries)
- Epithelial-Mesenchymal Transition
(drug effects)
- Female
- Fibrosis
- Fluorescent Antibody Technique
- Immunohistochemistry
- Kidney
(pathology)
- Kidney Tubules
(blood supply, drug effects, pathology)
- Microscopy, Electron, Transmission
- Rats
- Rats, Wistar
- Ureteral Obstruction
(complications)
- ortho-Aminobenzoates
(pharmacology)
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