Needle-free jet injection of small doses of Japanese encephalitis DNA and inactivated vaccine mixture induces neutralizing antibodies in miniature pigs and protects against fetal death and mummification in pregnant sows.

Abstract

Japanese encephalitis (JE) virus causes abortion and stillbirth in swine, and encephalitis in humans and horses. We have previously reported that immunogenicity of a DNA vaccine against JE was synergistically enhanced in mice by co-immunization with a commercial inactivated JE vaccine (JEVAX) under a needle-free injection system. Here, we found that this immunization strategy was also effective in miniature pigs. Because of the synergism, miniature pigs immunized twice with a mixture of 10 μg of DNA and a 1/100 dose of JEVAX developed a high neutralizing antibody titer (1:190 at 90% plaque reduction assay). Even using 1 μg of DNA, 3 of 4 pigs developed neutralizing antibodies. Following challenge, all miniature pigs with detectable neutralizing antibodies were protected against viremia. Pregnant sows inoculated with 10 or 1 μg of DNA mixed with JEVAX (1/100 dose) developed antibody titers of 1:40-1:320. Following challenge, fetal death and mummification were protected against in DNA/JEVAX-immunized sows.

• Authors: Jun-ichi Imoto, Tomohiro Ishikawa, Atsushi Yamanaka, Misako Konishi, Kenji Murakami, Tomoyuki Shibahara, Masanori Kubo, Chang-Kweng Lim, Masataka Hamano, Tomohiko Takasaki, Ichiro Kurane, Haruhide Udagawa, Yoshihiro Mukuta, Eiji Konishi
• Journal: Vaccine (Vaccine) Vol. 28 Issue 46 Pg. 7373-80 (Oct 28 2010) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 20851083 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Neutralizing
• Antibodies, Viral
• Japanese Encephalitis Vaccines
• Vaccines, DNA
• Vaccines, Inactivated

Topics

• Animals
• Antibodies, Neutralizing (blood)
• Antibodies, Viral (blood)
• Chlorocebus aethiops
• Encephalitis Virus, Japanese (immunology)
• Encephalitis, Japanese (immunology, prevention & control)
• Female
• Fetal Death (prevention & control, veterinary)
• Injections, Jet
• Insecta (cytology)
• Japanese Encephalitis Vaccines (administration & dosage, immunology)
• Pregnancy
• Swine
• Swine, Miniature
• Vaccines, DNA (administration & dosage, immunology)
• Vaccines, Inactivated (administration & dosage, immunology)
• Vero Cells
• Viremia (immunology, prevention & control)