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Phenylephrine induces early and late cardioprotection through mitochondrial permeability transition pore in the isolated rat heart.

AbstractBACKGROUND:
The aim of this study was to investigate the role of mitochondrial permeability transition pore (mPTP) in cardioprotection afforded by phenylephrine pretreatment in early and late phases.
METHODS:
Rat hearts were isolated and perfused with Krebs buffer in Langendorff preparation and subjected to 30 min regional ischemia followed by 60 min of reperfusion. Phenylephrine as a selective α1-adrenoceptor agonist and atractyloside as a specific opener of the mPTP were used. Seven groups (n = 6) of rats were randomly studied: (I) control: surgical procedure was performed with no ischemia/reperfusion, (II) ischemia/reperfusion: hearts underwent regional ischemia/reperfusion, (III) early phenylephrine: phenylephrine (50 μM) was perfused for 5 min prior to ischemia/reperfusion, (IV) late phenylephrine: rats were treated with phenylephrine (10 mg/kg, i.p) 24 h prior to ischemia/reperfusion, (V) early phenylephrine+atractyloside: hearts were perfused with phenylephrine as in group III and then atractyloside (20 mM) 5 min before reperfusion for 20 min, (VI) late phenylephrine+atractyloside: hearts were treated with phenylephrine as in group IV and then received atractyloside (20 mM), 5 min before reperfusion for 20 min, (VII) atractyloside-IR group: hearts were perfused with atractyloside (20 mM) 5 min before reperfusion for 20 min.
RESULTS:
Compared with ischemia/reperfusion group, perfusion of phenylephrine in early and late phases decreased myocardial infarct size (% of ischemia zone), reduced creatine kinase-MB (CK-MB) in the coronary effluent, and improved cardiac function. Administration of atractyloside abolished cardioprotective effects of phenylephrine in both early and late phases and returned infarct size, CK-MB and cardiac function to levels as seen in ischemia/reperfusion group.
CONCLUSION:
These results suggest that administration of atractyloside as a specific opener of the mPTP abolishes phenylephrine-induced early and late cardioprotection in the isolated rat hearts.
AuthorsRoya Naderi, Alireza Imani, Mahdieh Faghihi, Maryam Moghimian
JournalThe Journal of surgical research (J Surg Res) Vol. 164 Issue 1 Pg. e37-42 (Nov 2010) ISSN: 1095-8673 [Electronic] United States
PMID20850771 (Publication Type: Journal Article)
CopyrightCopyright © 2010 Elsevier Inc. All rights reserved.
Chemical References
  • Cardiotonic Agents
  • Phenylephrine
  • Creatine Kinase, MB Form
Topics
  • Animals
  • Cardiotonic Agents (pharmacology)
  • Coronary Circulation (drug effects, physiology)
  • Creatine Kinase, MB Form (blood)
  • Heart (drug effects)
  • Heart Rate (drug effects, physiology)
  • In Vitro Techniques
  • Ischemic Preconditioning, Myocardial (methods)
  • Male
  • Mitochondrial Membranes (drug effects, metabolism)
  • Myocardial Infarction (drug therapy, epidemiology, metabolism)
  • Myocardial Reperfusion Injury (drug therapy, epidemiology, metabolism)
  • Myocardium (metabolism)
  • Phenylephrine (pharmacology)
  • Rats
  • Rats, Wistar
  • Risk Factors
  • Ventricular Pressure (drug effects, physiology)

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