Abstract | OBJECTIVE: To investigate the expression of CD95 and special marker for activation of peripheral blood lymphocytes in patients with hand foot and mouth disease (HFMD) and its significance. METHODS: Immunofluorescent two-color flow cytometry was used to study the expression of CD95 and HLA-DR on lymphocytes in 58 patients with HFMD and 34 normal controls. RESULTS: Expression of CD3+ T cells was significantly lower in patients (63.82 +/- 7.74)% than that in controls (P < 0.001), meanwhile the expression of CD4+ T cells was (34.29 +/- 7.33)%, significantly lower than that of the controls (P < 0.005). The percentage of lymphocytes expressing HLA-DR in patients was (23.77 +/- 5.78)%, significantly higher than that of the controls (P < 0.005). Significant difference was observed in the expression of HLA- DR on CD8+ T cells in patients (1.34 +/- 1.12)% as compared with controls (P < 0.005). No significant difference in the expression of CD95 on lymphocytes was observed between patients and the controls (P > 0.05). CONCLUSION: The findings support that cellular immunodeficiency exists in patients and that lymphocytes were abnormally activated in the patients. The activation of peripheral blood T lymphocytes in patients mainly involves CD8 subset and it may play an important role in the immune response to antiviral infection.
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Authors | Ming-Li Zhu, Song-Ping Zhang, He-Qian Cao, Wei-Feng Liang, Jian-Hua Yu, Guo-Qiang Lou |
Journal | Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
(Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi)
Vol. 24
Issue 1
Pg. 33-5
(Feb 2010)
ISSN: 1003-9279 [Print] China |
PMID | 20848845
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Antigens
(genetics, immunology)
- CD4-Positive T-Lymphocytes
(immunology)
- Case-Control Studies
- Cells, Cultured
- Child, Preschool
- Hand, Foot and Mouth Disease
(genetics, immunology)
- Humans
- Infant
- Lymphocyte Count
- Male
- T-Lymphocyte Subsets
(immunology)
- fas Receptor
(genetics, immunology)
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