Abstract | AIMS AND BACKGROUND: METHODS AND STUDY DESIGN: We transfected melanoma antigen-1 (MAGE-1)-expressing T24 tumor cells with a plasmid encoding GPI-IL-2 and prepared the TEXs. Exosomes expressing GPI-IL-2 were characterized by electron microscope and Western blot analysis. RESULTS:
IL-2 was present on the cell surface in the GPI-anchored form as demonstrated by fluorescent microscope and ELISA analyses. Exosomes expressing GPI-IL-2 naturally contained bioactive GPI-IL-2 and tumor-associated antigen MAGE-1. Moreover, exosomes expressing GPI-IL-2-pulsed dendritic cells could induce the proliferation of T cells and the antigen-specific cytotoxic T-lymphocyte immune response more efficiently. CONCLUSIONS: GPI-IL-2 gene-modified tumor cells can make the TEXs contain GPI-IL-2, resulting in increased antitumor effects. Our study provided a feasible approach for exosome-based tumor immunotherapy.
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Authors | Jiamo Zhang, Yao Zhang, Chunli Luo, Yuguo Xia, Honglin Chen, Xiaohou Wu |
Journal | Tumori
(Tumori)
2010 May-Jun
Vol. 96
Issue 3
Pg. 452-9
ISSN: 0300-8916 [Print] United States |
PMID | 20845808
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cancer Vaccines
- Glycosylphosphatidylinositols
- Interleukin-2
- Interferon-gamma
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Topics |
- Blotting, Western
- Cancer Vaccines
(immunology)
- Cell Proliferation
(drug effects)
- Dendritic Cells
(drug effects, immunology)
- Exosomes
(genetics, immunology, metabolism)
- Gene Expression Regulation, Neoplastic
- Glycosylphosphatidylinositols
(metabolism)
- Humans
- Interferon-gamma
(metabolism)
- Interleukin-2
(metabolism)
- Lymphocyte Activation
(drug effects, immunology)
- Microscopy, Electron
- T-Lymphocytes, Cytotoxic
(drug effects, immunology)
- Urinary Bladder Neoplasms
(immunology, metabolism)
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