Quantitative determination of cefetamet in human plasma by liquid chromatography-mass spectrometry.

Abstract

Cefetamet is a potent antibiotic to treat respiratory and urinary tract infections. To improve oral bioavailability, it is administered as a prodrug, cefetamet pivoxyl hydrolyzed by esterase following absorption. A quantification method using a mass spectrometry was developed for the determination of cefetamet in human plasma. After a protein precipitation with acetonitrile, the analytes were chromatographed on a reversed-phase C₁₈ column and detected by a tandem mass spectrometer with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This method was used to measure the concentrations of the cefetamet in plasma after a single oral administration of 500 mg cefetamet pivoxyl.

• Authors: Keumhan Noh, Eunyoung Kim, Wonku Kang
• Journal: Biomedical chromatography : BMC (Biomed Chromatogr) Vol. 25 Issue 7 Pg. 779-82 (Jul 2011) ISSN: 1099-0801 [Electronic] England
• PMID: 20845371 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 John Wiley & Sons, Ltd.

Chemical References

• Prodrugs
• cefetamet
• Ceftizoxime
• cefetamet pivoxyl

Topics

• Adult
• Ceftizoxime (administration & dosage, analogs & derivatives, blood, pharmacokinetics)
• Chromatography, Reverse-Phase (methods)
• Drug Stability
• Humans
• Male
• Prodrugs (administration & dosage, pharmacokinetics)
• Reproducibility of Results
• Sensitivity and Specificity
• Spectrometry, Mass, Electrospray Ionization
• Tandem Mass Spectrometry (methods)
• Young Adult