Prooxidant action of carazolol in the Fenton-like reaction.

Abstract

Carazolol [4-(2-hydroxy-3-isopropyl-amino-propoxy)-carbazole], a β(3)-adrenoceptor agonist, is clinically used in the treatment of hypertension, cardiac arrhythmias and angina pectoris. Despite the beneficial effect of the drug, its high dose may contribute to cardiotoxicity. This study was conducted to examine whether carazolol can influence hydroxyl radical formation by a Fenton-like reaction [Co(II) + H(2)O(2) + HO(-)] in the presence of ethylenediaminetetraacetic acid. The oxygen free radicals and singlet oxygen ((1)O(2)) formation was traced by three different assay methods: chemiluminescence (CL), an electron spin resonance (ESR) spin trapping with 2,2,6,6-tetramethyl-4-piperidine and 5,5-dimethyl-1-pyrroline-1-oxide, and spectrophotometric determination of (1)O(2) based on bleaching of p-nitrosodimethylaniline. The effect of hydroxyl radical inhibitors and (1)O(2) quenchers on peroxidation of carazolol was also examined. The results indicated that carazolol enhanced the HO radical and (1)O(2) formation in a Fenton-like reaction.

• Authors: Irena Kruk, Teresa Michalska, Aleksandra Kładna, Hassan Y Aboul-Enein
• Journal: Luminescence : the journal of biological and chemical luminescence (Luminescence) 2011 Nov-Dec Vol. 26 Issue 6 Pg. 429-33 ISSN: 1522-7243 [Electronic] England
• PMID: 20842633 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 John Wiley & Sons, Ltd.

Chemical References

• Adrenergic beta-Antagonists
• Propanolamines
• Reactive Oxygen Species
• Spin Labels
• carazolol

Topics

• Adrenergic beta-Antagonists (chemistry)
• Electron Spin Resonance Spectroscopy
• Luminescence
• Propanolamines (chemistry)
• Reactive Oxygen Species (chemistry)
• Spin Labels