Abstract |
In this contribution, we have prepared and explored a novel nano-interface based probe for the rapid identification and highly sensitive detection of cancer cells by means of an electrochemical study. The new probe tetrathiafulvalene (TTF) carboxylate salt (TTF-(COONBu(4))(2), ditetrabutylammonium salt for propylenedithio-4',5'-tetrathiafulvalene-4,5-dicarboxylate), which has specific spectral and electrochemical properties, has been synthesized and assembled with carbon nanotubes to form a new type of nanocomposite. A simple method of fabricating the β-CD/MWCNT modified electrodes based on functionalized multi-walled carbon nanotubes (MWCNTs) and β- cyclodextrin (β-CD) has been explored by using glassy carbon electrodes (GCEs), which could remarkably enhance the sensitivity of the biomolecular detection. Our results demonstrate that the combination of the new probe TTF-(COONBu(4))(2) with β-CD/MWCNT modified electrodes could be readily utilized to sensitively detect cancer cells such as liver cancer cells SMMC-7721 and HepG2, drug sensitive leukemia K562/B.W cells and drug resistant leukemia K562/ADM cells, with a detection limit of ~10(3) cells mL(-1). This may provide a novel strategy for the potential and promising application of the new TTF molecular probe in the development of multi-signal responsive biosensors for the early diagnosis of cancers.
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Authors | Juan Zhao, Jianshi Jin, Chunhui Wu, Hui Jiang, Yanyan Zhou, Jinglin Zuo, Xuemei Wang |
Journal | The Analyst
(Analyst)
Vol. 135
Issue 11
Pg. 2965-9
(Nov 2010)
ISSN: 1364-5528 [Electronic] England |
PMID | 20838668
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Heterocyclic Compounds, 1-Ring
- Nanotubes, Carbon
- beta-Cyclodextrins
- Carbon
- betadex
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Topics |
- Carbon
(chemistry)
- Cells, Cultured
- Electrochemistry
- Electrodes
- Glass
(chemistry)
- Heterocyclic Compounds, 1-Ring
(chemical synthesis, chemistry)
- Humans
- Molecular Structure
- Nanotubes, Carbon
(chemistry)
- Neoplasms
(diagnosis, pathology)
- Particle Size
- Sensitivity and Specificity
- Stereoisomerism
- Surface Properties
- beta-Cyclodextrins
(chemistry)
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