Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: Global methylation state of genome DNA and lysine residues of histone H3 and H4 tails were assayed by Methylation by Isoschizomers (MIAMI) method and western blotting, respectively, in the presence or absence of OAZ expression. Ectopic expression of OAZ mediated hypomethylation of CpG islands of genome DNA and histone H3 lysine 9 dimethylation (H3K9me2). Protein level of DNA methyltransferase 3B (DNMT3B) and histone H3K9me specific methyltransferase G9a were down-regulated in OAZ transfectant. CONCLUSIONS/SIGNIFICANCE: OAZ induced hypomethylation of CpG islands of global genome DNA and H3K9me2 by down-regulating DNMT3B and G9a protein level. Hypomethylation of CpG islands of genome DNA and histone H3K9me2 is a potent mechanism of induction of the genes related to tumor suppression and DNA double strand break repair.
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Authors | Daisuke Yamamoto, Kaori Shima, Kou Matsuo, Takashi Nishioka, Chang Yan Chen, Guo-Fu Hu, Akira Sasaki, Takanori Tsuji |
Journal | PloS one
(PLoS One)
Vol. 5
Issue 9
Pg. e12554
(Sep 03 2010)
ISSN: 1932-6203 [Electronic] United States |
PMID | 20838441
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Histones
- Proteins
- ornithine decarboxylase antizyme
- DNA
- DNA (Cytosine-5-)-Methyltransferases
- DNA methyltransferase 3B
- Lysine
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Topics |
- Cell Line, Tumor
- DNA
(genetics, metabolism)
- DNA (Cytosine-5-)-Methyltransferases
(genetics, metabolism)
- DNA Methylation
- Histones
(genetics, metabolism)
- Humans
- Lysine
(genetics, metabolism)
- Methylation
- Mouth Neoplasms
(enzymology, genetics, metabolism)
- Proteins
(genetics, metabolism)
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