Abstract | OBJECTIVE: To generate a comprehensive map of the drug sensitivity of chronic lymphoid leukemia cells (CLL) using a newly developed in vitro drug-sensitivity assay based on automated evaluation of cell viability on single-cell level. MATERIALS AND METHODS: Primary CLL cells from 77 patients were tested using automated digital fluorescence microscopy. The effect of 27 frequently used chemotherapeutic agents was measured in short-term fluorescence survival assay. To avoid typical in vitro artifacts such as growth factor depletion and oxidative damage, the cell were cultured in a novel, total human blood lysate-based medium (OmniSanguine) in order to preserve the composition of growth factor flora and redox conditions of the in vivo environment. RESULTS: CLL cells from different patients showed considerable heterogeneity in their drug-sensitivity patterns. This pattern was stable even after in vitro activation of cell proliferation. Half of the samples were sensitive to fludarabine and chlorambucil. Daunorubicin was the most potent drug. It was effective in 75 of 77 cases. In addition, daunorubicin and prednisolone showed a strong synergistic effect. CONCLUSIONS:
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Authors | Henriette Skribek, Rita Otvos, Emilie Flaberg, Noemi Nagy, Laszlo Markasz, Staffan Eksborg, Tamas Masszi, Andras Kozma, Emma Adam, Attila Miseta, Eva Klein, Laszlo Szekely |
Journal | Experimental hematology
(Exp Hematol)
Vol. 38
Issue 12
Pg. 1219-30
(Dec 2010)
ISSN: 1873-2399 [Electronic] Netherlands |
PMID | 20837094
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Epirubicin
- Doxorubicin
- Prednisolone
- Daunorubicin
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Topics |
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Cell Cycle
(drug effects)
- Cluster Analysis
- Daunorubicin
(administration & dosage)
- Doxorubicin
(administration & dosage)
- Epirubicin
(administration & dosage)
- Humans
- Leukemia, Lymphocytic, Chronic, B-Cell
(drug therapy, pathology)
- Prednisolone
(administration & dosage)
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