Abstract |
Hepatocyte transplantation has been widely confirmed in the animal model experiments as an effective method for treatment of fulminant hepatic failure. However, the lack of donor organs remains a major problem. One solution is the development of transplantable hepatocytes. Herein we have transplanted intraperitoneally an established immortalized human fetal hepatic cell line (HL-7702) into CCl(4)-treated mice with acute liver injury to determine whether they provided life-saving metabolic support. The results showed lower levels of blood ammonia and higher content of liver albumin (P < .05) after HL-7702 transplantation versus nontransplanted controls at days 3 and 7. Histologic examination showed the transplantation group to be less affected at day 7 with no difference at day 14. In conclusion, an established immortal human fetal hepatic cell line may be a promising cell source providing life-saving metabolic support as a bioartificial liver device for the treatment of acute liver injury.
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Authors | Y B Yan, H Song, B S Zhong, Z Y Wang, S J Ying, F Wang |
Journal | Transplantation proceedings
(Transplant Proc)
Vol. 42
Issue 7
Pg. 2782-5
(Sep 2010)
ISSN: 1873-2623 [Electronic] United States |
PMID | 20832587
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | 2010. Published by Elsevier Inc. |
Chemical References |
- Serum Albumin
- Carbon Tetrachloride
- Aspartate Aminotransferases
- Alanine Transaminase
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Topics |
- Alanine Transaminase
(blood)
- Animals
- Aspartate Aminotransferases
(blood)
- Carbon Tetrachloride
(toxicity)
- Carbon Tetrachloride Poisoning
(surgery)
- Cell Line
- Fetus
- Hepatocytes
(pathology, transplantation)
- Humans
- Liver
(drug effects, pathology)
- Liver Failure, Acute
(surgery)
- Liver, Artificial
(statistics & numerical data)
- Mice
- Serum Albumin
(metabolism)
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