Ultrasensitive mycotoxin detection by STING sensors.

Abstract

Signal transduction by ion nano-gating (STING) technology is a label-free biosensor capable of identifying DNA and proteins. Based on a functionalized quartz nanopipette, the STING sensor includes specific recognition elements for analyte discrimination based on size, shape and charge density. A key feature of this technology is that it does not require any nanofabrication facility; each nanopipette can be easily, reproducibly, and inexpensively fabricated and tailored at the bench, thus reducing the cost and the turnaround time. Here, we show that STING sensors are capable of the ultrasensitive detection of HT-2 toxin with a detection limit of 100 fg/ml and compare the STING capabilities with respect to conventional sandwich assay techniques.

• Authors: Paolo Actis, Olufisayo Jejelowo, Nader Pourmand
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 26 Issue 2 Pg. 333-7 (Oct 15 2010) ISSN: 1873-4235 [Electronic] England
• PMID: 20829024 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Mycotoxins

Topics

• Biosensing Techniques (instrumentation)
• Conductometry (instrumentation)
• Equipment Design
• Equipment Failure Analysis
• Food Analysis (instrumentation)
• Food Contamination (analysis)
• Microchemistry (instrumentation)
• Mycotoxins (analysis)
• Nanostructures (chemistry, ultrastructure)
• Nanotechnology (instrumentation)