Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.

Abstract

Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino-acid sequence similarity, glucansucrases are classified as members of glycoside hydrolase family 70 (GH 70). Data on the crystal structure of GH 70 glucansucrases have yet to be reported. Here, the GH 70 glucansucrase GTF-SI from S. mutans was overexpressed in Escherichia coli strain BL21 (DE3), purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Orthorhombic GTF-SI crystals belonging to space group P2(1)2(1)2 were obtained. A diffraction data set was collected to 2.1 A resolution.

• Authors: Keisuke Ito, Sohei Ito, Tatsuro Shimamura, Yasuaki Kawarasaki, Keiko Abe, Takumi Misaka, Takuya Kobayashi, So Iwata
• Journal: Acta crystallographica. Section F, Structural biology and crystallization communications (Acta Crystallogr Sect F Struct Biol Cryst Commun) Vol. 66 Issue Pt 9 Pg. 1086-8 (Sep 01 2010) ISSN: 1744-3091 [Electronic] England
• PMID: 20823533 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycosyltransferases
• alternansucrase

Topics

• Crystallization
• Crystallography, X-Ray
• Glycosyltransferases (chemistry)
• Streptococcus mutans (enzymology)