Abstract | BACKGROUND: The birth of a boy is significantly more common than a girl prior to secondary recurrent miscarriage (SRM) and is associated with a poorer chance of a subsequent live birth. Children born after SRM are more likely to be girls. High-titer antisera specific for male antigens (H-Y) have been shown to arrest development of male bovine embryos efficiently. We consequently questioned the role of H-Y antibodies in women with SRM. METHODS: Serum samples from patients with unexplained SRM (n = 84), unexplained primary recurrent miscarriage (PRM) (n = 12) and healthy women (n = 37) were obtained. The samples were taken during pregnancy (gestational weeks 4-5) for 77 (80%) of the patients. Enzyme-linked immunosorbent assay was used to detect immunoglobulin G antibodies that specifically recognized any of the five recombinant H-Y proteins (EIF1AY, RPS4Y1, ZFY, DDX3Y and UTY) and their H-X homologs. RESULTS: H-Y-specific antibodies were more frequent in SRM patients (46%) compared with female controls (19%, P = 0.004) and PRM patients (8%, P = 0.01). The presence of H-Y antibodies in early pregnancy was associated with a low male: female birth ratio among the subsequent live births, as only 12% of children born to H-Y antibody-positive patients were boys compared with 44% boys born to H-Y antibody negative patients (P = 0.03). CONCLUSIONS: The high frequency of H-Y antibody-positive SRM patients and the association between the presence of these antibodies in early pregnancy and the low number of male offspring, suggest that maternal immune responses against H-Y antigens can cause pregnancy losses. Further exploring these mechanisms may increase our understanding of unexplained SRM.
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Authors | H S Nielsen, F Wu, Z Aghai, R Steffensen, A G van Halteren, E Spierings, O B Christiansen, D Miklos, E Goulmy |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 25
Issue 11
Pg. 2745-52
(Nov 2010)
ISSN: 1460-2350 [Electronic] England |
PMID | 20823116
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- H-Y Antigen
- H-Y antibody
- Immunoglobulins, Intravenous
- Isoantibodies
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Topics |
- Abortion, Habitual
(immunology)
- Adolescent
- Adult
- Enzyme-Linked Immunosorbent Assay
- Female
- H-Y Antigen
(immunology)
- Humans
- Immunoglobulins, Intravenous
(therapeutic use)
- Isoantibodies
(analysis)
- Male
- Pregnancy
- Pregnancy Outcome
- Pregnancy Trimester, First
- Sex Ratio
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