Effects of benazepril on cardiac fibrosis in STZ-induced diabetic rats.

Abstract

OBJECTIVE:

The present study was designed to explore the roles of MMP-2/TIMP-2 in cardiac fibrosis and to study the effects of benazepril, an angiotensin-converting enzyme inhibitor (ACEI) on cardiac remodelling in streptozotocin(STZ)-induced diabetic rats.

METHODS AND RESULTS:

Male Wistar rats were randomly divided into three groups: a normal control group (NC), a diabetes mellitus-untreated group (DM) and a diabetes mellitus benazepril-treated group (DB). Diabetes mellitus was induced in the DM and DB groups by intraperitoneal injection of streptozotocin (60 mg/kg). DB rats were treated with benazepril 10 mg/kg/day for 12 weeks by remedial perfusing of the stomach. In the DM group, compared with the NC group, the gene and protein expression of MMP-2 decreased while the TIMP-2 gene and protein expression increased in heart tissues, along with a markedly cardiac collagen deposition.All the above changes were attenuated by benazepril treatment in the DB group.

CONCLUSIONS:

The imbalance of MMP-2 and TIMP-2 expressions in heart tissues might participate in interstitial fibrosis in diabetic myocardiopathy. Benazepril may ameliorate cardiac fibrosis partly by regulating the MMP-2/TIMP-2 system.

• Authors: Qian Li, Yi Wang, Shu-zhen Sun, Yong-jie Tian, Ming-hua Liu
• Journal: Acta cardiologica (Acta Cardiol) Vol. 65 Issue 4 Pg. 431-9 (Aug 2010) ISSN: 0001-5385 [Print] England
• PMID: 20821936 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiotensin-Converting Enzyme Inhibitors
• Benzazepines
• Blood Glucose
• Tissue Inhibitor of Metalloproteinase-2
• Streptozocin
• Collagen
• Matrix Metalloproteinase 2
• benazepril

Topics

• Analysis of Variance
• Angiotensin-Converting Enzyme Inhibitors (pharmacology)
• Animals
• Benzazepines (pharmacology)
• Blood Glucose (metabolism)
• Body Weight
• Collagen (metabolism)
• Diabetes Mellitus, Experimental (drug therapy)
• Fibrosis (drug therapy)
• Immunoenzyme Techniques
• Male
• Matrix Metalloproteinase 2 (metabolism)
• Myocardium (pathology)
• Organ Size
• Random Allocation
• Rats
• Rats, Wistar
• Reverse Transcriptase Polymerase Chain Reaction
• Staining and Labeling
• Streptozocin
• Tissue Inhibitor of Metalloproteinase-2 (metabolism)