Sulfated modification and cytotoxicity of Portulaca oleracea L. polysaccharides.

Abstract

A water-soluble polysaccharide (POP1) was isolated from Portulaca oleracea L. Four sulfated derivatives of POP1 (POP1-s1, POP1-s2, POP1-s3 and POP1-s4) were prepared by chlorosulfonic acid method with N,N-Dicyclohexylcarbodiimide (DCC) as a dehydration-condensation agent. FT-IR spectra and 13C NMR spectra indicated the sulfated groups had been introduced at the C-6 and C-2 positions of POP1. Sulfated derivatives had different degree of substitution (DS) ranging from 1.01 to 1.81, and different weight-average molecular mass (Mw) ranging from 41.4 to 48.5 KDa. Sulfated derivatives except POP1-s5 inhibited the growth of HepG2 cells and Hela cells in vitro significantly, which indicated that sulfated modification could enhance cytotoxicity of POP1 on tumor cells. Flow cytometric studies revealed that sulfated derivatives could mediate the cell-cycle arrest of Hela cells in the S phase.

• Authors: Tong Chen, Jin Wang, Yuanyuan Li, Jianmin Shen, Ting Zhao, Haixia Zhang
• Journal: Glycoconjugate journal (Glycoconj J) Vol. 27 Issue 6 Pg. 635-42 (Aug 2010) ISSN: 1573-4986 [Electronic] United States
• PMID: 20820911 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Polysaccharides
• Sulfur

Topics

• Cell Cycle (drug effects)
• Cell Death (drug effects)
• Cell Proliferation (drug effects)
• Cell Shape (drug effects)
• HeLa Cells
• Hep G2 Cells
• Humans
• Magnetic Resonance Spectroscopy
• Polysaccharides (metabolism, pharmacology)
• Portulaca (chemistry)
• Spectroscopy, Fourier Transform Infrared
• Sulfur (metabolism)