Decreased chaperone activity of alpha-crystallins in naphthalene-induced cataract possibly results from C-terminal truncation.
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| Abstract |
Naphthalene-induced cataract has been extensively used to test potential anticataract drugs. Because the morphology as well as the toxic manifestations of naphthalene-induced cataract are reported to be similar to that of age-related cataract, naphthalene cataractogenesis in rats has been used as a valuable animal model to study the aetiology of age-related cataract in humans. This study aimed to determine whether the molecular chaperone activity of the alpha-crystallins was altered in naphthalene-induced cataract, and to clarify the possible mechanism for these changes. The data showed that the chaperone activity of the alpha-crystallins decreased in naphthalene-induced cataract. By mass spectrometry, C-terminal truncation of 16 amino acids and other post-translational modifications such as acetylation, phosphorylation, oxidation and carbamylation of the alpha-crystallins were detected. Furthermore, the results suggested that, at the proteomics level, naphthalene-induced cataract is a valuable animal model for the study of age-related cataract in humans.
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| Authors | Y Chen, L Yi, G Q Yan, Y X Jang, Y W Fang, X H Wu, X W Zhou, L M Wei |
| Journal | The Journal of international medical research (J Int Med Res) 2010 May-Jun Vol. 38 Issue 3 Pg. 1016-28 ISSN: 0300-0605 [Print] England |
| PMID | 20819438 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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