Effect of docetaxel on expression of eIF3a in human lung cancer A549 cell line.

Abstract

OBJECTIVE:

To explore the dose-dependent and time-dependent effect of docetaxel on the expression of mammalian eukaryotic initiation factor 3 subunit A (eIF3a) in lung cancer cell line.

METHODS:

The human lung cancer cell line A549 was treated with gradient concentrations of docetaxel for different time. Real-time PCR and Western blot were used to detect mRNA and protein expression levels of eIF3a and alpha-tubulin, respectively.

RESULTS:

Docetaxel did not affect alpha-tubulin expression at either mRNA level or protein level. When A549 cells were treated with high concentration of docetaxel (30 μg/L), the expression level of eIF3a mRNA tended to increase in a time-dependent manner. Protein expression level of alpha-tubulin was not associated with eIF3a expression significantly in cells treated by docetaxel.

CONCLUSION:

Docetaxel could slightly increase the expression of eIF3a mRNA, and eIF3a does not regulate the expression of alpha-tubulin in A549 cells treated by docetaxel.

• Authors: Zhicheng Gong, Xiaojing Xu, Ruiying Hou, Yue Guo, Feifeng Sheng, Dongsheng Ouyang, Honghao Zhou, Zhaoqian Liu
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 35 Issue 8 Pg. 771-6 (Aug 2010) ISSN: 1672-7347 [Print] China
• PMID: 20818067 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• EIF3A protein, human
• Eukaryotic Initiation Factor-3
• RNA, Messenger
• Taxoids
• Tubulin
• Docetaxel

Topics

• Antineoplastic Agents (pharmacology)
• Cell Line, Tumor
• Docetaxel
• Eukaryotic Initiation Factor-3 (genetics, metabolism)
• Humans
• Lung Neoplasms (pathology)
• RNA, Messenger (genetics, metabolism)
• Taxoids (pharmacology)
• Tubulin (genetics, metabolism)