Abstract | BACKGROUND: To investigate the mechanisms of multidrug resistance of brain tumors, to identify the site of cellular expression of P-gp in human brains in situ and to morphologically determine whether an association may exist between P-gp and caveolin-1. METHODS: RESULTS: High expression of P-gp was detected in vessel walls and the tissue surrounding the vessels. However, expression of P-gp was low in tumor cells. The expression of the other 4 multidrug resistance proteins was not observed in the vessel walls. Laser scanning microscopy showed P-gp and caveolin-1 co-expression: the two proteins co-localized either in the luminal endothelial compartment or at the border of the luminal/abluminal compartments. CONCLUSION: Chemotherapeutics drugs are interrupted in the end-feet of neuroepithelial cells of the BBB by P-gp, which weakens the chemotherapeutic effect. P-gp marks the BBB, and the transporter is localized in the luminal endothelial compartment where it co-localizes with caveolin-1.
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Authors | Zhenhua Guo, Jin Zhu, Lihua Zhao, Qing Luo, Xianqing Jin |
Journal | Journal of experimental & clinical cancer research : CR
(J Exp Clin Cancer Res)
Vol. 29
Pg. 122
(Sep 05 2010)
ISSN: 1756-9966 [Electronic] England |
PMID | 20815915
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ABCB1 protein, human
- ATP Binding Cassette Transporter, Subfamily B
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- CAV1 protein, human
- Caveolin 1
- Multidrug Resistance-Associated Proteins
- Vault Ribonucleoprotein Particles
- major vault protein
- GSTP1 protein, human
- Glutathione S-Transferase pi
- DNA Topoisomerases, Type II
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Topics |
- ATP Binding Cassette Transporter, Subfamily B
- ATP Binding Cassette Transporter, Subfamily B, Member 1
(analysis)
- Blood-Brain Barrier
(chemistry)
- Brain Neoplasms
(chemistry, drug therapy, pathology)
- Caveolin 1
(analysis)
- Child
- Child, Preschool
- China
- DNA Topoisomerases, Type II
(analysis)
- Drug Resistance, Neoplasm
- Endothelial Cells
(chemistry)
- Female
- Glutathione S-Transferase pi
(analysis)
- Humans
- Immunohistochemistry
- Infant
- Male
- Microscopy, Confocal
- Multidrug Resistance-Associated Proteins
(analysis)
- Neoplasm Staging
- Neuroglia
(chemistry)
- Vault Ribonucleoprotein Particles
(analysis)
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