[Norcantharidin inhibits DNA replication initiation protein Cdc6 in cancer cells].

Abstract

OBJECTIVE:

To explore the inhibitory effect of norcantharidin (NCTD) on the expression of DNA replication initiation protein Cdc6 in cancer cells.

METHODS:

MTT assay was performed to detect the inhibitory effect on different cancer cell lines, including HeLa, HepG2, Jurkat and Ramos cells. The effect of NCTD on Cdc6 protein level was detected by Western blotting, and BrdU incorporation assay was used to evaluate the DNA replication of the cells.

RESULTS:

NCTD significantly inhibited the proliferation of the cells and caused degradation of Cdc6 protein to result in the inhibition of the DNA replication of the cells shown by BrdU incorporation assay.

CONCLUSION:

NCTD can induce the degradation of Cdc6 in cancer cells to produce an anti-cancer effect.

• Authors: Jin-long Li, Yu-chen Cai, Zhi-ming Hu, Ji-min Gao
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 30 Issue 8 Pg. 1851-3 (Aug 2010) ISSN: 1673-4254 [Print] China
• PMID: 20813683 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Bridged Bicyclo Compounds, Heterocyclic
• CDC6 protein, human
• Cell Cycle Proteins
• Nuclear Proteins
• norcantharidin

Topics

• Bridged Bicyclo Compounds, Heterocyclic (pharmacology)
• Cell Cycle Proteins (metabolism)
• Cell Line, Tumor
• DNA Replication (drug effects)
• Humans
• Nuclear Proteins (metabolism)