Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: Extracts of Andrographis paniculata Nees are used for various ethnomedical conditions including hyperglycemia and hypertension complications. AIM OF THE STUDY: MATERIALS AND METHODS: RESULTS: Our data suggest that addition of compounds AP1 or AP2 reduces the phenotypes indicating diabetic nephropathy in MES-13 cells. The compound AP2 showed potent activity than AP1 in the reduction of apoptosis marker caspase-3, fibrosis marker TGF-β, and PAI-1. Furthermore, AP1 and AP2 do not have antioxidant ability in acellular environment; however, addition of AP1 and AP2 reduced intracellular oxidative states in high glucose cultured MES-13 cells. CONCLUSION: This is the first report on anti- diabetic nephropathy effect of AP1 and AP2 in part due to the regulation of intracellular signaling transduction, not mere clearance of reactive oxygen species. Thus, this study may be useful for drug development or food supplement for diabetes and nephropathy from Andrographis paniculata.
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Authors | Meng-Jen Lee, Yerra Koteswara Rao, Keru Chen, Yi-Ching Lee, Yi-Shuan Chung, Yew-Min Tzeng |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 132
Issue 2
Pg. 497-505
(Nov 11 2010)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 20813180
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- 14-deoxy-11,12-didehydroandrographolide
- Diterpenes
- Fibronectins
- Plant Extracts
- Plasminogen Activator Inhibitor 1
- Reactive Oxygen Species
- Transforming Growth Factor beta
- andrographolide
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Topics |
- Andrographis
- Animals
- Apoptosis
(drug effects)
- Cell Line, Transformed
- Diabetic Nephropathies
(drug therapy, metabolism, pathology)
- Diterpenes
(pharmacology)
- Fibronectins
(metabolism)
- Fibrosis
- Hyperglycemia
(complications)
- Mesangial Cells
(drug effects, physiology)
- Mice
- Oxidation-Reduction
(drug effects)
- Oxidative Stress
(drug effects)
- Plant Components, Aerial
- Plant Extracts
(pharmacology)
- Plasminogen Activator Inhibitor 1
(metabolism)
- Reactive Oxygen Species
(metabolism)
- Transforming Growth Factor beta
(metabolism)
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