Abstract |
The IL-13Ralpha2 gene encodes for a 65 kDa protein that forms one of the subunits of the interleukin-13 (IL-13) receptor. This gene is highly expressed in various types of human tumors including malignant gliomas. The expression level of IL-13Ralpha2 was examined in a total of 45 tissue samples of anaplastic astrocytomas (AAs) World Health Organization (WHO) grade III, glioblastomas (GBMs) WHO grade IV, and first-recurrent glioblastomas (frGBMs) after treatment with radiation and chemotherapy. IL-13Ralpha2 expression was detected by semiquantitative reverse transcription real-time polymerase chain reaction (PCR) using ABI PRISM 7700 and Qiagen QuantiTect SYBR Green PCR kits. The expression level of IL-13Ralpha2 (15 fold) was significantly reduced in frGBMs compared to the primary GBMs (p = 0.014), and significantly reduced by more than 15 fold (p = 0.003) in all untreated malignant astrocytomas (AAs and GBMs) compared with treated frGBMs. Expression of IL-13Ralpha2 seems to be lower in frGBMs compared to GBMs. The promising antitumor effect of IL-13 cytotoxin could be greatly reduced in frGBM or only achievable with higher amounts of cytotoxin, due to the significantly lower expression of the cytotoxin's target structure.
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Authors | Oliver Bozinov, Jens-Martin Kalk, Niklaus Krayenbühl, Christoph Michael Woernle, Ulrich Sure, Helmut Bertalanffy |
Journal | Neurologia medico-chirurgica
(Neurol Med Chir (Tokyo))
Vol. 50
Issue 8
Pg. 617-21
( 2010)
ISSN: 1349-8029 [Electronic] Japan |
PMID | 20805641
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Actins
- Exotoxins
- IL13-PE38
- Immunotoxins
- Interleukin-13
- Interleukin-13 Receptor alpha2 Subunit
- Recombinant Fusion Proteins
- Nimustine
- RNA
- Teniposide
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Topics |
- Actins
(genetics, metabolism)
- Adult
- Aged
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Astrocytoma
(metabolism, pathology, therapy)
- Brain Neoplasms
(metabolism, pathology, therapy)
- Exotoxins
(therapeutic use)
- Female
- Gene Expression Regulation, Neoplastic
(drug effects)
- Glioblastoma
(metabolism, pathology, therapy)
- Humans
- Immunotoxins
(therapeutic use)
- Interleukin-13
(therapeutic use)
- Interleukin-13 Receptor alpha2 Subunit
(genetics, metabolism)
- Male
- Middle Aged
- Neoplasm Recurrence, Local
(metabolism, pathology)
- Nimustine
(administration & dosage)
- RNA
(analysis)
- Recombinant Fusion Proteins
- Teniposide
(administration & dosage)
- Young Adult
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