Regulatory T cell defect in APECED patients is associated with loss of naive FOXP3(+) precursors and impaired activated population.

Abstract

The pathogenetic mechanisms of organ-specific autoimmune diseases remain obscured by the complexity of the genetic and environmental factors participating in the breakdown of tolerance. A unique opportunity to study the pathogenesis of human autoimmunity is provided by autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED), a rare inherited autoimmune disease caused by mutations in Autoimmune Regulator (AIRE) gene. Loss of AIRE function disrupts the deletion of autoreactive T cells and impairs the suppressive function of regulatory T (Treg) cells. Here we show by multiparameter flow cytometry that in healthy controls the peripheral naive Treg cell subset forms a slowly dividing, persistent reservoir of recent thymic emigrants (RTEs). In APECED patients the RTE Treg cells show accelerated turnover and shift to the activated pool and the RTE reservoir is depleted. Moreover, the activated Treg cell population in the patients expresses significantly less Forkhead box protein P3 (FOXP3) than in the healthy controls, consistent with the impairment of peripheral activation. Our results indicate that in addition to their thymic effects, loss-of-function mutations in AIRE disrupt the peripheral homeostasis and activation of Treg cells. This may synergize with failed negative selection to cause APECED.

• Authors: Sini M Laakso, Tuisku-Tuulia Laurinolli, Laura H Rossi, Anni Lehtoviita, Heikki Sairanen, Jaakko Perheentupa, Eliisa Kekäläinen, T Petteri Arstila
• Journal: Journal of autoimmunity (J Autoimmun) Vol. 35 Issue 4 Pg. 351-7 (Dec 2010) ISSN: 1095-9157 [Electronic] England
• PMID: 20805020 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier Ltd. All rights reserved.

Chemical References

• FOXP3 protein, human
• Forkhead Transcription Factors
• Transcription Factors

Topics

• Adult
• Cell Separation
• DNA Mutational Analysis
• Down-Regulation
• Female
• Flow Cytometry
• Forkhead Transcription Factors (biosynthesis, genetics)
• Homeostasis
• Humans
• Lymphocyte Activation (genetics)
• Male
• Middle Aged
• Mutation (genetics)
• Polyendocrinopathies, Autoimmune (genetics, immunology)
• Precursor Cells, T-Lymphoid (immunology, metabolism, pathology)
• T-Lymphocytes, Regulatory (immunology, metabolism, pathology)
• Transcription Factors (genetics, immunology, metabolism)
• AIRE Protein