Abstract |
Multi-walled carbon-nanotubes (MWCNTs)-induced apoptotic changes were studied in human lung epithelium cell line-A549. Non-cytotoxic doses of MWCNTs were identified using tetrazolium bromide salt (MTT) and lactate dehydrogenase (LDH) release assays. Cells were exposed to MWCNTs (0.5-100 μg/ml) for 6-72 h. Internalization and characterization of CNTs was performed by electron microscopy. Apoptotic changes were estimated by nuclear condensation, DNA laddering, and confirmed by expression of associated markers: p(53), p(21WAF1/CIP1), Bax, Bcl(2) and activated caspase-3. MWCNTs induced the production of reactive oxygen species and malondialdehyde along with significant decrease in the activity of catalase and glutathione. MWCNTs-induced ROS generation was found not to be associated with the mitochondrial activity. In general, the changes were significant at 10 and 50 μg/ml only. Results indicate the involvement of oxidative stress and apoptosis in A549 cells exposed to MWCNTs. Our studies provide insights of the mechanisms involved in MWCNTs-induced apoptosis at cellular level.
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Authors | Ritesh K Srivastava, Aditya B Pant, Mahendra P Kashyap, Vivek Kumar, Mohathshim Lohani, Ludwig Jonas, Qamar Rahman |
Journal | Nanotoxicology
(Nanotoxicology)
Vol. 5
Issue 2
Pg. 195-207
(Jun 2011)
ISSN: 1743-5404 [Electronic] England |
PMID | 20804439
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Nanotubes, Carbon
- Reactive Oxygen Species
- Uncoupling Agents
- Rotenone
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Topics |
- Animals
- Apoptosis
(drug effects)
- Biomarkers
(metabolism)
- Cell Line, Tumor
(drug effects)
- Humans
- Lung Neoplasms
- Mitochondria
(metabolism)
- Nanotubes, Carbon
(adverse effects, ultrastructure)
- Oxidative Stress
(drug effects)
- Reactive Oxygen Species
(metabolism)
- Rotenone
(metabolism)
- Uncoupling Agents
(metabolism)
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