Proteomic profile regulated by the anticancer peptide CIGB-300 in non-small cell lung cancer (NSCLC) cells.

Abstract

CIGB-300 is a proapoptotic peptide-based drug that abrogates the CK2-mediated phosphorylation. This peptide has antineoplastic effect on lung cancer cells in vitro and in vivo. To understand the mechanisms involved on such anticancer activity, the NCI-H125 cell line proteomic profile after short-term incubation (45 min) with CIGB-300 was investigated. As determined by 2-DE or 2D-LC-MS/MS, 137 proteins changed their abundances more than 2-fold in response to the CIGB-300 treatment. The expression levels of proteins related to ribosome biogenesis, metastasis, cell survival and proliferation, apoptosis, and drug resistance were significantly modulated by the presence of CIGB-300. The protein translation process was the most affected (23% of the identified proteins). From the proteome analysis of the NCI-H125 cell line, novel potentialities for CIGB-300 as anticancer agent were evidenced.

• Authors: Arielis Rodríguez-Ulloa, Yassel Ramos, Jeovanis Gil, Yasser Perera, Lila Castellanos-Serra, Yairet García, Lázaro Betancourt, Vladimir Besada, Luis J González, Jorge Fernández-de-Cossio, Aniel Sanchez, Joem M Serrano, Hernán Farina, Daniel F Alonso, Boris E Acevedo, Gabriel Padrón, Alexis Musacchio, Silvio E Perea
• Journal: Journal of proteome research (J Proteome Res) Vol. 9 Issue 10 Pg. 5473-83 (Oct 01 2010) ISSN: 1535-3907 [Electronic] United States
• PMID: 20804217 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Peptides, Cyclic
• Proteome
• CIGB-300

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Non-Small-Cell Lung (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Chromatography, Liquid
• Cluster Analysis
• Electrophoresis, Gel, Two-Dimensional
• Humans
• Lung Neoplasms (metabolism, pathology)
• Mass Spectrometry
• Peptides, Cyclic (pharmacology)
• Protein Biosynthesis (drug effects)
• Proteome (analysis, classification)
• Proteomics (methods)