Protective effect of carnosol on lung injury induced by intestinal ischemia/reperfusion.

Abstract

PURPOSE:

Carnosol is a phenolic diterpene that has potent antioxidant and anti-inflammatory activities. The purpose of this study was to investigate the preconditioning effects of carnosol on lung injury induced by intestinal ischemia/reperfusion (II/R).

METHODS:

Rats were divided into control, II/R, and carnosol groups. The II/R model was established by clamping the superior mesenteric artery for 1 h and reperfusion at 2, 4, and 6 h after ischemia. The carnosol group received 3 mg/kg carnosol intraperitoneally 1 h before the operation. The rats were then euthanized, and blood and lung specimens were obtained for analysis.

RESULTS:

The II/R induced lung injury, characterized by histological changes and significant increasing of bronchoalveolar lavage fluid protein. The activity of lung tissue superoxide was weakened, the tissue myeloperoxidase activity and serum interleukin-6 level increased significantly in II/R groups. A strong positive expression of lung intercellular adhesion molecule-1 (ICAM-1) and nuclear factor kappa B (NF-kappaB) were observed. Pretreatment with carnosol markedly reduced lung injury by increasing the tissue superoxide activity and decreasing the myeloperoxidase activity and interleukin-6 level, which was parallel to the decreased expression of ICAM-1 and NF-kappaB.

CONCLUSION:

Carnosol was able to ablate lung injury induced by II/R, partly attributed to the inhibition of NF-kappaB activation.

• Authors: Xiao-Feng Tian, Ji-Hong Yao, Xue-Song Zhang, Shu-Sen Zheng, Xin-Hua Guo, Li-Ming Wang, Zhen-Zhen Wang, Ke-Xin Liu
• Journal: Surgery today (Surg Today) Vol. 40 Issue 9 Pg. 858-65 (Sep 2010) ISSN: 1436-2813 [Electronic] Japan
• PMID: 20740350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Abietanes
• Interleukin-6
• NF-kappa B
• Plant Extracts
• Intercellular Adhesion Molecule-1
• carnosol
• Peroxidase
• Superoxide Dismutase

Topics

• Abietanes (therapeutic use)
• Acute Lung Injury (etiology, metabolism, pathology, prevention & control)
• Animals
• Bronchoalveolar Lavage Fluid (chemistry)
• Intercellular Adhesion Molecule-1 (metabolism)
• Interleukin-6 (blood)
• Ischemic Preconditioning
• Lung (metabolism, pathology)
• Male
• NF-kappa B (antagonists & inhibitors, metabolism)
• Peroxidase (metabolism)
• Plant Extracts (therapeutic use)
• Rats
• Rats, Wistar
• Reperfusion Injury (complications)
• Rosmarinus
• Superoxide Dismutase (metabolism)