A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea.

Abstract

A laccase, with HIV-1 reverse transcriptase inhibitory activity (IC(50)=12.7 μM) and antiproliferative activity against HepG2 cells (IC(50)=5.6 μM) and MCF7 cells (IC(50)=6.5 μM), was purified from fresh fruiting bodies of the edible white common Agrocybe cylindracea mushroom. The laccase, which had a novel N-terminal sequence, displayed a molecular mass of 58 kDa within the range reported for most other mushroom laccases. The purification protocol entailed ion exchange chromatography on DEAE-cellulose, SP-Sepharose, and Q-Sepharose and gel filtration on Superdex 75. The laccase was adsorbed on DEAE-cellulose and Q-Sepharose, but unadsorbed on SP-Sepharose. Its optimum pH was pH 3-4 and its optimum temperature was 50°C. The activity of the isolated laccase differed from one substrate to another. The ranking was ABTS>N,N-dimethyl-1,4-phenylenediamine>hydroquinone>catechol>2-methylcatechol>pyrogallol.

• Authors: D D Hu, R Y Zhang, G Q Zhang, H X Wang, T B Ng
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 18 Issue 5 Pg. 374-9 (Mar 15 2011) ISSN: 1618-095X [Electronic] Germany
• PMID: 20739163 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier GmbH. All rights reserved.

Chemical References

• Laccase
• HIV Reverse Transcriptase

Topics

• Agrocybe (enzymology)
• Amino Acid Sequence
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Chromatography, Gel
• Chromatography, Ion Exchange
• Electrophoresis, Polyacrylamide Gel
• Fruiting Bodies, Fungal (enzymology)
• HIV Reverse Transcriptase (antagonists & inhibitors)
• Humans
• Hydrogen-Ion Concentration
• Laccase (chemistry, isolation & purification, metabolism, pharmacology)
• Molecular Weight
• Sequence Homology, Amino Acid
• Substrate Specificity
• Temperature