Abstract | OBJECTIVES: METHODS: A contaminated open fracture was created in 32 Brown Norway rats. A comminuted femoral fracture was created, followed by crushing, and the 5-mm bone defect was inoculated with Staphylococcus aureus (10 colony-forming units/mL) and Escherichia coli (10 colony-forming units/mL). The scaffold was stabilized in the defect with an intramedullary Kirschner wire. Gentamicin was loaded onto the scaffolds at two doses, either 10 mg (n = 12) or 20 mg (n = 10). Controls (n = 10) received no antibiotics. All three groups had 10 microg bone morphogenetic protein loaded on the scaffold. Serial radiographs were obtained. Microbiologic analysis, microcomputed tomography, and histology were performed. RESULTS: There was a statistically significant difference in the radiographic evidence of osteomyelitis (P = 0.004) and callus formation (P = 0.021) between the treated and control groups. Bone culture analysis results were not significant for S. aureus (P = 0.29) or E. coli (P = 0.25). There was no difference in the mean scaffold volume or density of the three treatment groups. CONCLUSIONS:
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Authors | Rena L Stewart, Joseph T Cox, David Volgas, James Stannard, Lynn Duffy, Ken B Waites, Tien-Min Chu |
Journal | Journal of orthopaedic trauma
(J Orthop Trauma)
Vol. 24
Issue 9
Pg. 587-91
(Sep 2010)
ISSN: 1531-2291 [Electronic] United States |
PMID | 20736800
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Drug Carriers
- Gentamicins
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Topics |
- Absorbable Implants
- Animals
- Anti-Bacterial Agents
(administration & dosage)
- Disease Models, Animal
- Drug Carriers
- Femoral Fractures
(drug therapy, microbiology)
- Fracture Healing
(drug effects, physiology)
- Fractures, Open
(drug therapy, microbiology)
- Gentamicins
(administration & dosage)
- Osteomyelitis
(drug therapy, microbiology)
- Rats
- Rats, Inbred BN
- Tissue Scaffolds
- Treatment Outcome
- Weight-Bearing
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