Abstract | INTRODUCTION: Gas6, the protein product of the growth arrest specific gene 6, is present in human circulation at subnanomolar concentrations. It is secreted by endothelial cells and is important for the activation of endothelium during inflammation. Axl, the tyrosine kinase receptor for Gas6, is also present in endothelium and can be cleaved and released into the circulation. The soluble of form Axl (sAxl), which is present in plasma, can bind Gas6 and inhibit Axl-mediated cell signalling. METHODS: We have developed reproducible and accurate enzyme-linked immunosorbent assays for both Gas6 and sAxl and used them to investigate plasma samples from 70 patients with severe sepsis, 99 patients with sepsis, 42 patients with various infections causing fever but no systemic inflammatory response syndrome (SIRS), 20 patients with SIRS without verified infection, and 100 blood donors that served as controls. Correlations between Gas6 and sAxl concentrations and other commonly used analytes were investigated. RESULTS: CONCLUSIONS: We can confirm results of earlier studies showing that circulating Gas6 is increased in sepsis and related syndromes. sAxl is increased, but less pronounced than Gas6. The concentrations of Gas6 and sAxl correlate with a number of inflammatory markers, suggesting a role in systemic inflammation.
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Authors | Carl Ekman, Adam Linder, Per Akesson, Björn Dahlbäck |
Journal | Critical care (London, England)
(Crit Care)
Vol. 14
Issue 4
Pg. R158
( 2010)
ISSN: 1466-609X [Electronic] England |
PMID | 20731857
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Intercellular Signaling Peptides and Proteins
- Proto-Oncogene Proteins
- growth arrest-specific protein 6
- Receptor Protein-Tyrosine Kinases
- Axl Receptor Tyrosine Kinase
- AXL protein, human
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Topics |
- Biomarkers
(blood)
- Case-Control Studies
- Enzyme-Linked Immunosorbent Assay
- Humans
- Infections
(blood, immunology)
- Intercellular Signaling Peptides and Proteins
(blood)
- Proto-Oncogene Proteins
(blood)
- Receptor Protein-Tyrosine Kinases
(blood)
- Sepsis
(blood, immunology)
- Systemic Inflammatory Response Syndrome
(blood, immunology)
- Axl Receptor Tyrosine Kinase
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