Human papillomavirus in ameloblastoma.

Abstract

OBJECTIVE:

Ameloblastomas are benign epithelial tumors of odontogenic origin, with a high recurrence rate and local aggressiveness. A few preliminary studies have demonstrated HPV presence mainly in peripheral ameloblastomas. The purpose of this study was to detect HPV-DNA in intraosseous ameloblastomas.

METHODS:

Eighteen cases of intraosseous ameloblastomas of different histological variants were selected. Immunohistochemistry, CISH, nested-PCR, and INNOLiPA HPV Genotyping v2 were used.

RESULTS:

The predominant age group was between the third and fourth decades of life. Males were more affected with 61% and females represented 39%. Of the 18 cases, 7 were solid multicystic tumors and 11 were unicystic. Of the histological variants, the plexiform represented 3 (17%) of the 18, 2 (11%) were follicular, 2 (11%) were acanthomatous, and 1 (6%) was desmoplastic. All cases were HPV negative by immunohistochemistry and CISH. HPV-DNA was detected in 6 (33%) of the cases by nested-PCR. HPV 6 was the most frequent genotype in 4 (66%) of the 6. Two cases presented a mixture of HPV 16, 33, and HPV 6, 42 respectively. Four of the unicystic ameloblastomas were HPV positive; of these, all presented koilocytic changes and were associated with dentigerous cysts, whereas only 2 positive cases corresponded to solid ameloblastomas. None of the positive cases were related to recurrence rate.

CONCLUSIONS:

We may conclude that HPV low and high risk was detected in our sample of intraosseous ameloblastomas. HPV positivity was observed more in the unicystic cases than solid types.

• Authors: Maria Correnti, Marcello Rossi, Maira Avila, Marianella Perrone, Helen Rivera
• Journal: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (Oral Surg Oral Med Oral Pathol Oral Radiol Endod) Vol. 110 Issue 3 Pg. e20-4 (Sep 2010) ISSN: 1528-395X [Electronic] United States
• PMID: 20727492 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright (c) 2010 Mosby, Inc. All rights reserved.

Chemical References

• DNA, Viral

Topics

• Adolescent
• Adult
• Aged
• Alphapapillomavirus (classification, isolation & purification)
• Ameloblastoma (complications, pathology, virology)
• Cysts (complications, pathology, virology)
• DNA, Viral (analysis)
• Female
• Humans
• Male
• Mandibular Neoplasms (complications, pathology, virology)
• Middle Aged
• Papillomavirus Infections (complications, diagnosis)
• Retrospective Studies
• Young Adult