Abstract | BACKGROUND: MATERIALS AND METHODS: Skin biopsy specimens from abdominal wall incisions were obtained during surgery from patients with direct inguinal hernia, indirect inguinal hernia or without hernia (controls). MMP-2, MT-1 MMP and TIMP-2 expression were determined using immunocytochemistry and immunoblotting in intact tissue and in cultured fibroblasts isolated from the biopsies. The degradation activity of MMP-2 was semiquantitatively determined using zymography. RESULTS: Significantly greater active MMP-2 expression was observed in skin fibroblasts obtained from patients with direct hernia compared with controls. MT1-MMP expression was directly correlated with MMP-2 expression with most intense staining produced in patients with direct or indirect inguinal hernia. TIMP-2, was maximally expressed in the control group, with significantly diminished expression levels recorded in the hernia groups. CONCLUSIONS: Our findings indicate active MMP-2 upregulation in the abdominal skin of patients with direct inguinal hernia. This metalloproteinase plays a role in matrix degradation, weakening the abdominal wall. Skin disorders and previously described transversalis fascia defects in these patients could point to a systemic collagen metabolism abnormality as a risk factor for direct hernia.
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Authors | Gemma Pascual, Marta Rodríguez, Verónica Gómez-Gil, Cynthia Trejo, Julia Buján, Juan Manvel Bellón |
Journal | European journal of clinical investigation
(Eur J Clin Invest)
Vol. 40
Issue 12
Pg. 1113-21
(Dec 2010)
ISSN: 1365-2362 [Electronic] England |
PMID | 20718849
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2010 The Authors. European Journal of Clinical Investigation © 2010 Stichting European Society for Clinical Investigation Journal Foundation. |
Chemical References |
- Tissue Inhibitor of Metalloproteinase-2
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 14
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Topics |
- Abdomen
- Adult
- Biopsy
- Cells, Cultured
(metabolism)
- Female
- Fibroblasts
(metabolism)
- Hernia, Inguinal
(metabolism)
- Humans
- Immunoblotting
- Immunohistochemistry
- Male
- Matrix Metalloproteinase 14
(metabolism)
- Matrix Metalloproteinase 2
(metabolism)
- Middle Aged
- Skin
(metabolism, pathology)
- Tissue Inhibitor of Metalloproteinase-2
(metabolism)
- Young Adult
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