HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Active matrix metalloproteinase-2 upregulation in the abdominal skin of patients with direct inguinal hernia.

AbstractBACKGROUND:
Prior studies suggest impaired collagen metabolism involving the whole abdominal wall including the skin in patients with abdominal hernia. We compared expression patterns of matrix metalloproteinase-2 (MMP-2) and its modulators membrane type-1-matrix metalloproteinase (MT-1 MMP) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in the skin of patients with and without primary inguinal hernia.
MATERIALS AND METHODS:
Skin biopsy specimens from abdominal wall incisions were obtained during surgery from patients with direct inguinal hernia, indirect inguinal hernia or without hernia (controls). MMP-2, MT-1 MMP and TIMP-2 expression were determined using immunocytochemistry and immunoblotting in intact tissue and in cultured fibroblasts isolated from the biopsies. The degradation activity of MMP-2 was semiquantitatively determined using zymography.
RESULTS:
Significantly greater active MMP-2 expression was observed in skin fibroblasts obtained from patients with direct hernia compared with controls. MT1-MMP expression was directly correlated with MMP-2 expression with most intense staining produced in patients with direct or indirect inguinal hernia. TIMP-2, was maximally expressed in the control group, with significantly diminished expression levels recorded in the hernia groups.
CONCLUSIONS:
Our findings indicate active MMP-2 upregulation in the abdominal skin of patients with direct inguinal hernia. This metalloproteinase plays a role in matrix degradation, weakening the abdominal wall. Skin disorders and previously described transversalis fascia defects in these patients could point to a systemic collagen metabolism abnormality as a risk factor for direct hernia.
AuthorsGemma Pascual, Marta Rodríguez, Verónica Gómez-Gil, Cynthia Trejo, Julia Buján, Juan Manvel Bellón
JournalEuropean journal of clinical investigation (Eur J Clin Invest) Vol. 40 Issue 12 Pg. 1113-21 (Dec 2010) ISSN: 1365-2362 [Electronic] England
PMID20718849 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2010 The Authors. European Journal of Clinical Investigation © 2010 Stichting European Society for Clinical Investigation Journal Foundation.
Chemical References
  • Tissue Inhibitor of Metalloproteinase-2
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 14
Topics
  • Abdomen
  • Adult
  • Biopsy
  • Cells, Cultured (metabolism)
  • Female
  • Fibroblasts (metabolism)
  • Hernia, Inguinal (metabolism)
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Male
  • Matrix Metalloproteinase 14 (metabolism)
  • Matrix Metalloproteinase 2 (metabolism)
  • Middle Aged
  • Skin (metabolism, pathology)
  • Tissue Inhibitor of Metalloproteinase-2 (metabolism)
  • Young Adult

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: