Abstract | BACKGROUND: METHODS: RESULTS: A higher level of active nuclear-localized NF-kappaB was observed in the metastatic SCCHN specimens group (p < 0.01). The NF-kappaB activities of SCCHN cell lines with different metastatic potentials were then determined and in excellent agreement with results found in SCCHN specimens, highly metastatic SCCHN cell lines expressed high level of NF-kappaB activity. The treatment of highly metastatic SCCHN cells with NF-kappaB inhibitors reduced the in vitro cell invasion capacity of the cells without affecting the apoptotic rate. Additionally, the NF-kappaB inhibitors significantly inhibited the experimental lung metastasis of Tb cells and lymph node metastasis of TL cells in nude mice. Furthermore, the expression of metastasis-related proteins, such as matrix metalloproteinase 9 and vascular endothelial growth factor, was inhibited by pyrrolidine dithiocarbonate. CONCLUSIONS: This study suggests that NF-kappaB activity significantly contributes to tumor hematologic and lymphatic metastases and may aid in the development of early detection methods or therapies targeting non-conventional molecular targets.
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Authors | Ming Yan, Qin Xu, Ping Zhang, Xiao-jian Zhou, Zhi-yuan Zhang, Wan-tao Chen |
Journal | BMC cancer
(BMC Cancer)
Vol. 10
Pg. 437
(Aug 17 2010)
ISSN: 1471-2407 [Electronic] England |
PMID | 20716363
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BAY 11-7085
- NF-kappa B
- Nitriles
- RNA, Messenger
- Sulfones
- Luciferases
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Animals
- Apoptosis
- Blotting, Western
- Carcinoma, Squamous Cell
(drug therapy, metabolism, pathology)
- Cell Adhesion
- Cell Movement
- Cell Proliferation
- Electrophoretic Mobility Shift Assay
- Female
- Flow Cytometry
- Fluorescent Antibody Technique
- Head and Neck Neoplasms
(drug therapy, metabolism, pathology)
- Humans
- Immunoenzyme Techniques
- Luciferases
(metabolism)
- Lung Neoplasms
(drug therapy, metabolism, secondary)
- Lymphatic Metastasis
- Male
- Mice
- Mice, Inbred BALB C
- Middle Aged
- NF-kappa B
(antagonists & inhibitors, genetics, metabolism)
- Nitriles
(pharmacology)
- RNA, Messenger
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
- Sulfones
(pharmacology)
- Tumor Cells, Cultured
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