Efficacy, heat stability and safety of intranasally administered Bacillus subtilis spore or vegetative cell vaccines expressing tetanus toxin fragment C.

Abstract

Bacillus subtilis strains expressing tetanus toxin fragment C (TTFC) were tested as vaccine candidates against tetanus in adult mice. Mice received three intranasal (IN) exposures to 10(9) spores or 10(8) vegetative cells of B. subtilis expressing recombinant TTFC. Immunized mice generated protective systemic and mucosal antibodies and survived challenge with 2× LD(100) of tetanus toxin. Isotype analysis of serum antibody indicated a balanced Th1/Th2 response. Lyophilized vaccines stored at 45° C for ≥ 12 months, remained effective. Immunized conventional and SCID mice remained well, and no histological changes in brain or respiratory tract were detected. Lyophilized/reconstituted B. subtilis tetanus vaccines administered IN to mice appear safe, heat-stable, and protective against lethal tetanus challenge.

• Authors: Sangun Lee, Boris R Belitsky, David W Brown, James P Brinker, Kathryn O Kerstein, John E Herrmann, Gerald T Keusch, Abraham L Sonenshein, Saul Tzipori
• Journal: Vaccine (Vaccine) Vol. 28 Issue 41 Pg. 6658-65 (Sep 24 2010) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 20709005 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Bacterial
• Peptide Fragments
• Tetanus Toxin
• Tetanus Toxoid
• tetanus toxin fragment C

Topics

• Administration, Intranasal
• Animals
• Antibodies, Bacterial (blood)
• Bacillus subtilis (immunology)
• Base Sequence
• Female
• Freeze Drying
• Mice
• Mice, Inbred BALB C
• Mice, SCID
• Molecular Sequence Data
• Peptide Fragments (immunology)
• Tetanus (immunology, prevention & control)
• Tetanus Toxin (immunology)
• Tetanus Toxoid (immunology)