To study the antitumor effect of
phlomiol extracted from Phlomis younghusbandii in vivo and in vitro. The inhibitory effect of
phlomiol on two kinds of human
tumor cells proliferation was assayed by MTT method. Transplant
tumor models of S180 and H22 were used. After
transplantation, different doses of
phlomiol were given to the mice for 14 days. The inhibitory rates were calculated. MTT method was used to assess the proliferation of T spleen lymphocyte cells and the activity of NK cells in
tumor-bearing mice with S180.
Phlomiol (50-100 mg x L(-1)) inhibited the proliferation of three kinds of
tumor cells in vitro, antitumor effect of
phlomiol was in a dose-dependent manner (r = 0.989, P < 0.05). The inhibitory rates of
phlomiol (2.5, 5, 10 mg x kg(-1)) were 28.5%-65.0% and 35.0%-74.5% in
tumor-bearing mice with S180 and H22 respectively, It could stimulate the spleen T-cells in
tumor-bearing mice with S180 and increase the activity of the NK cells.
Phlomiol could inhibit the proliferation of three kinds of
tumor cells in vitro, present antitumor effect on the
tumor-bearing mice, and improve the immunological function.