Abstract | BACKGROUND: METHODS: Expression of survivin was analyzed in a collection of normal and malignant prostatic tissues by immunohistochemical staining. In vitro studies were conducted in PC-3M, C4-2B, and 22Rv1 prostate cancer cells. The effect of selenium on survivin expression was analyzed by Western blotting and semi-quantitative RT-PCR. Survivin gene knockdown was carried out by transfecting cells with a short hairpin RNA ( shRNA) designed against survivin. Cell proliferation was quantitated by the 3-(4,5-Dimethylthiazol-2-yl)- 2,5-Diphenyltetrazolium Bromide (MTT) assay and apoptosis by propidium iodide staining followed by flow cytometry analysis. Finally, in vivo tumor growth assay was performed by establishing PC-3M xenograft in nude mice and monitoring tumor growth following transfection and treatment. RESULTS: CONCLUSIONS:
Selenium could inhibit the growth of hormone-refractory prostate cancer cells both in vitro and in vivo, but the effects were modest. The growth inhibition was not mediated by downregulating survivin expression. Survivin silencing greatly enhanced the growth inhibitory effects of selenium.
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Authors | Xichun Liu, Ruijuan Gao, Yan Dong, Lifang Gao, Yanying Zhao, Lijuan Zhao, Xuejian Zhao, Haitao Zhang |
Journal | BMC cancer
(BMC Cancer)
Vol. 10
Pg. 418
(Aug 10 2010)
ISSN: 1471-2407 [Electronic] England |
PMID | 20698994
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Anticarcinogenic Agents
- Birc5 protein, mouse
- Inhibitor of Apoptosis Proteins
- Organoselenium Compounds
- RNA, Messenger
- Repressor Proteins
- Survivin
- Selenocysteine
- methylselenic acid
- Cysteine
- selenomethylselenocysteine
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Topics |
- Animals
- Anticarcinogenic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Blotting, Western
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cysteine
(analogs & derivatives, pharmacology)
- Down-Regulation
- Drug Synergism
- Gene Silencing
- Humans
- Immunoenzyme Techniques
- Inhibitor of Apoptosis Proteins
(antagonists & inhibitors, genetics, metabolism)
- Male
- Mice
- Mice, Nude
- Neoplasms, Hormone-Dependent
(drug therapy, genetics, pathology)
- Organoselenium Compounds
(pharmacology)
- Prognosis
- Prostatic Neoplasms
(drug therapy, genetics, pathology)
- RNA, Messenger
(genetics)
- Repressor Proteins
(antagonists & inhibitors, genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Selenocysteine
(analogs & derivatives)
- Survival Rate
- Survivin
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