Abstract | BACKGROUND: MATERIALS AND METHODS: RESULTS: Combination treatment using TRAIL and triptolide decreased cell viability in all cell lines at 48 h, with greater cell killing than that which was observed with either drug alone. This decrease in viability was associated with increases in annexin-V staining and caspase-3 activity. Western blot analysis demonstrated increases in PARP cleavage and decreases in XIAP expression that were dose-dependent. CONCLUSIONS: TRAIL and triptolide in combination decreased cell viability and enhanced apoptosis. Furthermore, Western blot analysis suggests that triptolide sensitizes cells to TRAIL-induced apoptotic cell death by inhibiting expression of XIAP, a protein known to inhibit apoptosis. Our results demonstrate that combination of TRAIL and triptolide enhance apoptosis in cholangiocarcinoma cell lines.
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Authors | Kimberly A Clawson, Daniel Borja-Cacho, Mara B Antonoff, Ashok K Saluja, Selwyn M Vickers |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 163
Issue 2
Pg. 244-9
(Oct 2010)
ISSN: 1095-8673 [Electronic] United States |
PMID | 20691980
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2010. Published by Elsevier Inc. |
Chemical References |
- Annexin A5
- Diterpenes
- Epoxy Compounds
- Phenanthrenes
- TNF-Related Apoptosis-Inducing Ligand
- X-Linked Inhibitor of Apoptosis Protein
- XIAP protein, human
- triptolide
- Poly(ADP-ribose) Polymerases
- Caspase 3
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Topics |
- Annexin A5
(analysis)
- Apoptosis
(drug effects)
- Bile Duct Neoplasms
(drug therapy, metabolism, pathology)
- Bile Ducts, Intrahepatic
- Caspase 3
(metabolism)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Cholangiocarcinoma
(drug therapy, metabolism, pathology)
- Diterpenes
(pharmacology)
- Drug Synergism
- Epoxy Compounds
(pharmacology)
- Humans
- Phenanthrenes
(pharmacology)
- Poly(ADP-ribose) Polymerases
(metabolism)
- TNF-Related Apoptosis-Inducing Ligand
(pharmacology)
- X-Linked Inhibitor of Apoptosis Protein
(metabolism)
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