Expression of matrix metalloproteinase-2 and tissue inhibitors of metalloproteinase-1 (TIMP-1, TIMP-2 and TIMP-3) in women with uterine prolapse but without urinary incontinence.

Abstract

OBJECTIVE:

To investigate the activities of matrix metalloproteinase-2 (MMP-2) and its inhibitors, tissue inhibitor of metalloproteinase-1, -2 and -3 (TIMP-1, TIMP-2 and TIMP-3), in the pelvic support and nonsupport tissue of women with uterine prolapse but without urinary incontinence.

STUDY DESIGN:

Paired samples of uterosacral ligament and cervical tissue were obtained from 11 postmenopausal and 8 premenopausal women with severe uterine prolapse. Nine premenopausal women without prolapse were selected as normal controls. Immunoreactivity of MMP-2 and TIMPs was demonstrated by immunohistochemistry. Steady state of MMP-2 as well as TIMPs messenger RNA (mRNA) expression was analyzed by polymerase chain reaction (PCR) with quantitative expression determined by multiplex PCR.

RESULTS:

A significantly higher expression of MMP-2 mRNA and lower expression of TIMP-2 mRNA were found in uterosacral ligament in uterine prolapse women compared to controls. In the cervical tissue, however, the MMP-2 and TIMPs mRNA expression was comparable between prolapse and control groups. With regard to menopausal status, there was no significant difference in MMP-2 and TIMPs mRNA expression between premenopausal and postmenopausal women with uterine prolapse.

CONCLUSIONS:

An increase in MMP-2 mRNA and a decrease in TIMP-2 mRNA expression in uterosacral ligament are related to uterine prolapse in women without urinary incontinence.

• Authors: Ching-Chung Liang, Hong-Yuan Huang, Ling-Hong Tseng, Shuenn-Dhy Chang, Tsia-Shu Lo, Chyi-Long Lee
• Journal: European journal of obstetrics, gynecology, and reproductive biology (Eur J Obstet Gynecol Reprod Biol) Vol. 153 Issue 1 Pg. 94-8 (Nov 2010) ISSN: 1872-7654 [Electronic] Ireland
• PMID: 20685029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010. Published by Elsevier Ireland Ltd.

Chemical References

• RNA, Messenger
• TIMP3 protein, human
• Tissue Inhibitor of Metalloproteinase-1
• Tissue Inhibitor of Metalloproteinase-3
• Tissue Inhibitor of Metalloproteinase-2
• Collagen
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Adult
• Aged
• Collagen (metabolism)
• Female
• Humans
• Matrix Metalloproteinase 2 (analysis, genetics)
• Matrix Metalloproteinase 9 (analysis, genetics)
• Middle Aged
• RNA, Messenger (analysis)
• Tissue Inhibitor of Metalloproteinase-1 (analysis, genetics)
• Tissue Inhibitor of Metalloproteinase-2 (analysis, genetics)
• Tissue Inhibitor of Metalloproteinase-3 (analysis, genetics)
• Urinary Incontinence (metabolism)
• Uterine Prolapse (metabolism)