Albumin fusion of thioredoxin--the production and evaluation of its biological activity for potential therapeutic applications.

Abstract	Thioredoxin-1 (Trx) is a redox-active protein with anti-inflammatory effects. The effect of albumin fusion on the pharmacokinetic and pharmacodynamic properties of Trx was evaluated in this study. The findings indicate that the properties of human serum albumin and the fusion protein are comparable. The fusion protein showed similar plasma concentration and organ distribution profiles as human serum albumin. The fusion protein accumulated in lungs, reaching levels higher than Trx. In an insulin reducing assay, the activity of the fusion protein was 60% of the activity of Trx. However, survival rate of endotoxic shock mice induced by the administration of a lipopolysaccharide and D-galactosamine for fusion protein was double that of Trx. The findings reported herein indicate that the fusion protein is likely to have great clinical applications in areas such as the treatment of reperfusion injuries.
Authors	Shotaro Ikuta, Victor Tuan Giam Chuang, Yu Ishima, Keisuke Nakajou, Masato Furukawa, Hiroshi Watanabe, Toru Maruyama, Masaki Otagiri
Journal	Journal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 147 Issue 1 Pg. 17-23 (Oct 01 2010) ISSN: 1873-4995 [Electronic] Netherlands
PMID	20678999 (Publication Type: Journal Article)
Copyright	Copyright © 2010. Published by Elsevier B.V. All rights reserved.
Chemical References	Anti-Inflammatory Agents, Non-Steroidal Recombinant Fusion Proteins Serum Albumin Thioredoxins
Topics	Animals Anti-Inflammatory Agents, Non-Steroidal (chemistry, pharmacokinetics, therapeutic use) Blotting, Western Circular Dichroism Disease Models, Animal Drug Stability Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Humans Male Mice Mice, Inbred Strains Pichia (genetics) Protein Conformation Recombinant Fusion Proteins (chemistry, pharmacokinetics, therapeutic use) Serum Albumin (chemistry, genetics) Shock, Septic (drug therapy) Survival Analysis Thioredoxins (administration & dosage, pharmacokinetics, therapeutic use) Tissue Distribution

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