Abstract |
The biological effects of a class of oxygenated imidazolium ionic liquids were studied in comparison with alkyl imidazolium salts (BMIM BF4 and BMIM N(CN)2).The cellular and subcellular effects were evaluated on rat pheochromocytoma PC12 cell lines, through MTT test, lactate dehydrogenase release and acetylcholinesterase inhibition; the eco-toxicological responses were assessed through the acute toxicity tests towards Daphnia magna and Vibrio fischeri. The introduction of ethoxy moieties in the lateral chain of imidazolium cations reduced the biological effects in all the tests. The acute toxicity towards D. magna was not affected by the number of ethoxy units, but the crustacean seemed to be sensitive to the type of anion; on the contrary, a further addition of ethoxy moieties increased the toxicity towards V. fischeri, M(OE)4MIM N(CN)2 being the most toxic oxygenated ionic liquid. In the cytotoxicity assays the salts with oxygenated cations resulted ineffective compared to BMIMs, independently from the anion and the number of ethoxy units in the lateral chain. In order to estimate the influence on membrane fluidity, an analysis of fluorescence anisotropy was done and it indicated that BMIM BF4, the most toxic ionic liquid among the tested ones, led to a destabilization of the model membranes at any molarity.
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Authors | Chiara Samorì, Danilo Malferrari, Paola Valbonesi, Adele Montecavalli, Fabio Moretti, Paola Galletti, Giorgio Sartor, Emilio Tagliavini, Elena Fabbri, Andrea Pasteris |
Journal | Ecotoxicology and environmental safety
(Ecotoxicol Environ Saf)
Vol. 73
Issue 6
Pg. 1456-64
(Sep 2010)
ISSN: 1090-2414 [Electronic] Netherlands |
PMID | 20674022
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright (c) 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Environmental Pollutants
- Imidazoles
- Ionic Liquids
- L-Lactate Dehydrogenase
- Acetylcholinesterase
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Topics |
- Acetylcholinesterase
(metabolism)
- Aliivibrio fischeri
(drug effects)
- Animals
- Cell Survival
(drug effects)
- Daphnia
(drug effects)
- Environmental Pollutants
(chemistry, toxicity)
- Imidazoles
(chemistry, toxicity)
- Ionic Liquids
(chemistry, toxicity)
- L-Lactate Dehydrogenase
(metabolism)
- Oxidation-Reduction
- PC12 Cells
- Rats
- Structure-Activity Relationship
- Toxicity Tests, Acute
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