[Effect and mechanism of methyl protodioscin in protecting cardiomyocytes against anoxia/reoxygenation injury].

Abstract

OBJECTIVE:

To study the effect and mechanism of methyl protodioscin (MPD), an active ingredients of yamogenin, in protecting cardiomyocytes (CMC) against anoxia/reoxygenation (A/R) injury.

METHODS:

Cultured CMCs of neonatal SD rats were randomly divided into three groups, cells in Group A were untreated normal cells, cells in Group B and C were made to injury CMC model by A/R, and only those in Group C were treated with MPD. Levels of ATPase activity and lactate dehydrogenase (LDH) in cell membrane of CMCs were determined. Besides, the mRNA expression of sodium-calcium exchanger (NCX) in MPD treated CMCs was detected.

RESULTS:

As compared with Group B, the degree of CMC injury was significantly milder and the activities of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase were higher in Group C after cells were treated with MPD in concentration of 10 microg/mL and 50 microg/mL. The mRNA expression of NCX in CMCs was down-regulated after MPD treatment (P < 0.05).

CONCLUSION:

MPD could maintain the low calcium internal environment in CMCs by way of protecting the membranous function of Na+ -pump and Ca2+ -pump, and influencing the Ca2+ transmembrane transportation in CMCs.

• Authors: Zong Ning, Yi-kui Li, Yan Zhou
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 30 Issue 4 Pg. 407-9 (Apr 2010) ISSN: 1003-5370 [Print] China
• PMID: 20669680 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Saponins
• methyl protodioscin
• Diosgenin
• yamogenin
• Oxygen

Topics

• Animals
• Cell Hypoxia
• Cells, Cultured
• Diosgenin (analogs & derivatives, pharmacology)
• Myocardial Reperfusion Injury (metabolism)
• Myocytes, Cardiac (drug effects, metabolism)
• Oxygen (adverse effects)
• Rats
• Rats, Sprague-Dawley
• Saponins (pharmacology)