Abstract |
The expression of parathyroid hormone-related protein ( PTHrP) in abnormal human parathyroids was investigated. Northern blot analysis of RNA extracted from human benign parathyroid adenomata (n = 4) revealed multiple PTHrP mRNA species ranging in size from 1.8 to 4 kb. The relative abundance of PTHrP mRNA expressed in two of the adenomata was similar to that of a tumour (DAF) associated with humoral hypercalcaemia of malignancy, whereas PTHrP mRNA was of low abundance in a third and was undetectable in the fourth. PTHrP-like immunoreactivity was detected in extracts of abnormal parathyroid tissue (benign adenoma (n = 7), hyperplasia (n = 5) and parathyroid carcinoma (n = 2] using a sensitive specific two-site immunoradiometric assay for human (h) PTHrP(1-86) and a radioimmunoassay for hPTHrP(1-34). Ratios of hPTHrP(1-86)- and hPTHrP(1-34)-like immunoreactivities relative to hPTH(1-84)-like immunoreactivity in the parathyroid tissue extracts were, on average, less than 1%. PTHrP bioactivity in the extracts could not be distinguished from that of PTH, by an osteosarcoma cell bioassay. We conclude that, despite reports of over-expression of PTHrP mRNA in parathyroid adenomata, the potential contribution of PTHrP to the total PTH-like activity of adenomata and other abnormal parathyroid tissue may be insignificant relative to PTH.
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Authors | H M Docherty, W A Ratcliffe, D A Heath, K Docherty |
Journal | The Journal of endocrinology
(J Endocrinol)
Vol. 129
Issue 3
Pg. 431-8
(Jun 1991)
ISSN: 0022-0795 [Print] England |
PMID | 2066698
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neoplasm Proteins
- PTHLH protein, human
- Parathyroid Hormone
- Parathyroid Hormone-Related Protein
- Peptide Fragments
- Peptides
- Proteins
- RNA, Messenger
- parathyroid hormone-related protein (1-86)
- parathyroid hormone-related protein (1-34)
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Topics |
- Adenoma
(genetics)
- Blotting, Northern
- Carcinoma
(genetics)
- Gene Expression
(physiology)
- Humans
- Hyperplasia
- Neoplasm Proteins
(genetics)
- Parathyroid Diseases
(genetics)
- Parathyroid Glands
(chemistry, pathology)
- Parathyroid Hormone
(analysis, genetics)
- Parathyroid Hormone-Related Protein
- Peptide Fragments
(analysis)
- Peptides
(analysis)
- Proteins
(analysis, genetics)
- RNA, Messenger
(analysis)
- Radioimmunoassay
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