Chromic-acid formaldehyde fixation of nucleic acids of bacteriophage phi6 and infectious bovine rhinotracheitis virus.

Abstract	Bacteriophage phi6 nucleic acid was present as a torus after chromic acid-formaldehyde-OSO4 fixation and acetone and propylene oxide dehydration. A herpes virus, infectious bovine rhinotracheitis virus, had its DNA mostly as a torus, collapsed in the centre, or as a network, after glutaraldehyde-OSO4 fixation, but in an uncollapsed torus or network formation after chromic acid-formaldehyde-OSO4. This fixative stabilized nucleic acids, allowing acetone dehydration and plastic embedding without collapse of nucleic acid to the centre of the virion.
Authors	W G Langenberg, R L Sharpee
Journal	The Journal of general virology (J Gen Virol) Vol. 39 Issue 2 Pg. 377-80 (May 1978) ISSN: 0022-1317 [Print] England
PMID	206662 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References	DNA, Viral Fixatives RNA, Viral Chromium Formaldehyde Osmium Glutaral
Topics	Chromium Coliphages (ultrastructure) DNA, Viral (analysis) Fixatives Formaldehyde Glutaral Herpesvirus 1, Bovine (ultrastructure) Osmium RNA, Viral (analysis)

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