Kinsenoside isolated from Anoectochilus formosanus suppresses LPS-stimulated inflammatory reactions in macrophages and endotoxin shock in mice.

Abstract

In the present study, we reported that kinsenoside, a major component of Anoectochilus formosanus, inhibited inflammatory reactions in mouse peritoneal lavage macrophages and protects mice from endotoxin shock. In LPS-stimulated mouse peritoneal lavage macrophages, kinsenoside inhibited the inflammatory mediators, such as nitric oxide, TNF-[alpha], IL-1[beta], monocyte chemoattractant protein 1, and macrophage migration inhibitory factor production. Furthermore, kinsenoside decreased the formation of a nuclear factor [kappa]B-DNA complex and nuclear p65 and p50 protein levels. Kinsenoside inhibited nuclear factor [kappa]B translocation through both I[kappa]B[alpha]-dependent and -independent pathway. In contrast, it stimulated anti-inflammatory cytokine IL-10 generation and enhanced the mRNA expression of IL-10 and suppressor of cytokine signaling 3 in the same cells induced by LPS. In an animal model, both pretreatment and posttreatment of kinsenoside increased the survival rate of ICR mice challenged by LPS (80 mg/kg, i.p.). Pretreatment with kinsenoside decreased serum levels of TNF-[alpha], IL-1[beta], IL-10, monocyte chemoattractant protein 1, and migration inhibitory factor at 1 h after sublethal dose of LPS (40 mg/kg, i.p.) in mice. In contrast, kinsenoside enhanced serum IL-10 level at 24 h after LPS injection in mice. In conclusion, kinsenoside inhibited the production of inflammatory mediators and enhanced anti-inflammatory cytokine generation. Therefore, kinsenoside can alleviate acute inflammatory hazards.

• Authors: Hung-Bo Hsiao, Jin-Bin Wu, Ho Lin, Wen-Chuan Lin
• Journal: Shock (Augusta, Ga.) (Shock) Vol. 35 Issue 2 Pg. 184-90 (Feb 2011) ISSN: 1540-0514 [Electronic] United States
• PMID: 20661184 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3-glucopyranosyloxybutanolide
• Cytokines
• Inflammation Mediators
• Lipopolysaccharides
• Monosaccharides
• NF-kappa B
• 4-Butyrolactone

Topics

• 4-Butyrolactone (analogs & derivatives)
• Animals
• Cytokines (metabolism)
• Inflammation Mediators (metabolism)
• Lipopolysaccharides (pharmacology)
• Macrophages, Peritoneal (metabolism)
• Magnoliopsida (chemistry)
• Male
• Mice
• Mice, Inbred ICR
• Monosaccharides (chemistry, pharmacology)
• NF-kappa B (metabolism)
• Shock, Septic (chemically induced, drug therapy, metabolism)