Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: The effects of whole formula and its herbal main ingredients on rat MSCs differentiation towards cardiomyocytes were investigated and the distinct protein expression profile in MSC-derived cardiomyocytes studied. MATERIALS AND METHODS: The protein expression profiles of rat MSCs and cardiomyocyte-like cells were determined with two-dimensional gel electrophoresis (2-DE). RESULTS: Our data demonstrated that SLF can induce MSCs into cardiomyocyte-like cells and around 36 proteins mainly involved in cytoskeleton, cell tissue energy metabolism and signal transduction have been shown to be regulated distinctly by SLF treatment. CONCLUSIONS: Data presented in this study suggest that large rearrangement of the proteome occur during the differentiation process of the MSCs to terminally differentiated cardiomyocyte-like cells and offer the possibility for further characterization of specific targets driving the stem cell differentiation.
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Authors | Xuemei Fan, Xue Li, Shufeng Lv, Yiming Wang, Yanfeng Zhao, Guoan Luo |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 131
Issue 3
Pg. 575-80
(Oct 05 2010)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 20659544
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Drugs, Chinese Herbal
- Proteins
- shuanglong
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Topics |
- Animals
- Cell Differentiation
(drug effects)
- Cytoskeleton
(drug effects, metabolism)
- Drugs, Chinese Herbal
(pharmacology)
- Electrophoresis, Gel, Two-Dimensional
- Energy Metabolism
(drug effects)
- Mesenchymal Stem Cells
(drug effects, metabolism)
- Myocytes, Cardiac
(drug effects, metabolism)
- Proteins
(drug effects, metabolism)
- Proteomics
(methods)
- Rats
- Rats, Sprague-Dawley
- Signal Transduction
(drug effects)
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